|Desired Industry: Biotech
|Desired Job Location: Chicago, Illinois
||Date Posted: 6/11/2006
|Type of Position: Full-Time Permanent
||U.S. Work Authorization: Yes
|Job Level: Experienced with over 2 years experience
||Willing to Travel: Yes, 25-50%
|Highest Degree Attained: Doctoral
||Willing to Relocate: Yes
Industrial Experience: Worked for two years in industry, in the area of natural drug discovery, strain improvement, process modification, scale up (from 20 L to 1KL and 50 KL reactor) for production of Echinocandin, Rifamycin and Lovastatin. Plant worked with, was following c-GMP, GLP, and had US-FDA approval. Supervised group of six people and was also effective in inter-departmental & cross functional activities, technology transfer, writing SOP, monthly reports, technical reports, & project proposals.
Postdoctoral Experience: Four and half years postdoctoral experience involving metabolic engineering for production of biofuel-ethanol, lactic acid using E.coli. Worked on enzyme structure- function relationship, using tools like cloning, over-expression, purification, site directed mutation, enzyme kinetics, SDS-PAGE, Western blot, 2-D gel analysis. Working on functional genomics aspects of metal recovery and metal resistance by using various tools of molecular biology, micro-array and proteomics.
Postdoctoral Research Associate: Department of Biological Sciences, Purdue University Lilly Hall of Life Sciences, West Lafayette, IN-47907. Also working at Oak Ridge National Laboratory for the project (Since Oct. 05).
Elucidating the molecular basis and regulation of Chromium (VI), resistance to metal toxicity using integrated biochemical, genomic and proteomic approaches.
Cloned, over expressed genes for DNA-binding response regulator as well as putative azoreductase, purification of proteins, and establishment their roles in regulation of Chromium (VI) reduction and resistance.
Transcriptome and proteome analysis of the Shewanella oneidensis MR-1 under different physiological conditions.
Postdoctoral Fellow: Department of Chemistry & Biochemistry, Miami University, Hughes Laboratory, Oxford, Ohio, 45056 (Oct. 03 to Sept. 05).
Enzyme structure function relationship
Developed structurefunction model for pyrroline-5-carboxylate reductase using bioinformatic approach.
Cloned gene proC gene, over expressed, purified recombinant enzyme, site-directed mutagenesis & chemical modifications of the enzyme to prove the model.
Developed activity based Targeted Proteomic assay for detection of the enzyme on 2-D protein gels.
Postdoctoral Associate: Dept. Microbiology & Cell Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL-32611 (Sept 01-June 03).
Metabolic engineering of E.coli for fermentative production of lactic acid and ethanol.
Strain improvement of genetically engineered ethanolgenic E. coli to increase alcohol tolerance and ethanol production.
Comparison of ethanol production patterns using different pentoses and hexoses.
Cloning of sucrose utilization genes into genetically engineered E. coli for demonstrating production of lactic acid from molasses and sucrose in complex and minimal medium.
Executive R & D: Lupin Chemicals Ltd., INDIA (June00 -Sept.01)
Plan, carry out, supervise (group of six officers) involved in R & D activity.
Evaluation and standardization of novel molecular biology, genetic engineering techniques for improving Rifamycin B productivity in shake flask and lab scale fermentor.
Characterization and identification of enzymes involved in antibiotic production.
Process modification to increase Rifamycin and Lovastatin yield at 50 KL capacity fermentor. Scale up of process from lab scale to pilot plant and production scale reactor.
Preparation, modification, and authorization of Standard Operating Procedure (SOP) for different activities in R & D and pilot plant and to educate staff about GLP and GMP in the plant.
Preparation of scientific reports, monthly reports of R&D activities. Submission of projects proposal for production novel products on commercial scale. Involved in the technology transfer and commercialization of lovastatin production.
Research Scientist: Nicholas Piramal India Ltd, Quest Institute of Life Sciences, Mumbai (Formerly Hoechst Research Center) (Nov. 99- June 00).
Process development for anti-fungal antibiotic through bench & pilot scale using new isolates.
Trouble-shooting and optimization of industrial processes.
Development of biochemical technologies and commercialization of proprietary biochemical technologies.
Gathering scientific and technical literatures, submission of new projects, product and process technologies, compilation and evaluation of project technical reports and feasibility reports.
PhD (Microbiology): Department of Chemical Technology (UDCT), University of Mumbai, India. Research work involved application of different aspects of fermentation, biochemical engineering, process development, downstream processing, & scale up for biocatalysis of benzaldehyde to L-phenylacetylcarbinol (L-PAC) using yeast. (Oct.95-Oct. 99).
Research resulted nine publications and also leads to develop interest by several industries for commercialization of process for Ephedrine production.
MS (Microbiology): Department of Life sciences, North Maharashtra University, Jalgaon India. (June 92- May 94).
Association for Microbiology
Well acquainted with preparation of gene libraries, cloning of genes, sequencing, site-directed mutagenesis, over-expression, purification of recombinant enzymes, enzyme kinetics.
Micro-array and proteomic analysis under different physiological conditions.
Isolation, identification of proteins, characterization of current and emerging technologies, proteomics, biotransformation and process improvement & development of commercial strains, assessment of drug substances, effectiveness of the drug, understanding metabolic mechanism of drugs, application of biomarkers.
Designing of biotransformation processes at laboratory scale fermentor and scaling it up to pilot plant & production scale for pharmaceutical compounds.
Hands on experience with the Chemap, New Brunswick & Sartorious fermenters, Bubble column reactors, Internal loop reactors, Ultracentrifuge, Ultrafiltration unit, Supercritical Fluid Extraction Unit, Microwave oven, ABI Prism 310 Genetic Analyzer, Bio-Rad Versa Doc Imaging system.
Well acquainted with various analytical techniques including GLC, GC-MS, HPLC, Chiral HPLC, Polarimeter, HPTLC, UV, FT-IR and NMR, ELISA, CD spectro-polarimeter, MALDI-TOF, Mass Spectrophotometer.
Available upon request.
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