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Scientist Resume


Desired Industry: Education/Teaching/Training SpiderID: 57640
Desired Job Location: Johnson City, Tennessee Date Posted: 2/7/2012
Type of Position: Full-Time Permanent Availability Date:
Desired Wage:
U.S. Work Authorization: Yes
Job Level: Management (Manager, Director) Willing to Travel: Yes, Less Than 25%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Experience:
2009-Present Postdoctoral Fellow
Neurocardiology Laboratory
Department of Physiology, James H. Quillen College of Medicine,
East Tennessee State University, Johnson City, TN 37614
Currently my research is focused on understanding the central neural chemotransduction signaling of angina during cardiac ischemia by studying the relationship between spinal substance P release and the TRPV1 receptor in a rodent model.

2008-2009 Adjunct Professor of Biology
Department of Biology
Virginia Highlands Community College, Abingdon, VA 24212
My responsibilities included lectures and laboratories for General Biology and Anatomy and Physiology.

2008-2009 Laboratory Director
Anderson Compounding Pharmacy
Bristol, TN 37620
My responsibilities included participating in the daily production of safe and effective compounded medicine. I was also responsible for implementing the use of the PK software that monitors inventory, allows the creation of formula worksheets, and keeps files of physicians, wholesalers, pharmacists and technicians.

2004-2008 Graduate Assistant
Department of Biochemistry and Molecular Biology, James H. Quillen College of Medicine,
East Tennessee State University, Johnson City, TN 37614
My research included the investigation of the Heat Shock Protein A12B (HspA12B) gene and its role in angiogenesis. We studied its effect on angiogenesis by up and down-regulating HspA12B expression in in vitro and in vivo angiogenesis assays. We also determined the mechanism by which HspA12B regulates angiogenesis by identifying its interacting proteins through yeast-2-hybrid screening and confirmed the interaction through co-immunoprecipitation and co-localization.

2001-2004 Research Specialist, Level 5
Department of Pathology, James H. Quillen College of Medicine,
East Tennessee State University, Johnson City, TN 37614
I worked on determining the role of the p-53 induced gene CGR19 in apoptosis. I performed various apoptosis assays using cell culture, in vitro assays and flow cytometry.

1997-2000 Research Assistant
Schepens Eye Research Institute, an Affiliate of Harvard Medical School,
Boston, MA 02114
I was responsible for studying androgenís control on gene expression in rabbit meibomian and lacrimal glands through the use of differential display. The technique involved the treatment of the rabbits through the use of androgen eye drops prior to dissection of the glands and mRNA isolation. RT-PCR and subsequent comparison of DNA expression between the control and treatment groups was then performed. Cryosections of the glands were also prepared for in situ hybridization.



Education:
Doctor of Philosophy in Biomedical Science with a concentration in Biochemistry and Molecular Biology (2008)
East Tennessee State University, Quillen College of Medicine,
Department: Biochemistry and Molecular Biology, Johnson City, TN
Dissertation: Characterization of HspA12B as a Novel Angiogenesis Regulator
Supervisor: Dr. Zhihua Han, Ph.D.

Master of Science in Biochemistry (1993)
Albany Medical College, Albany, NY
Department: Biochemistry and Molecular Biology
Master Thesis: Role of Sulfhydryl/Disulfide Exchange in Endothelin-Receptor Interactions
Supervisor: Dr. Thomas T. Andersen, Ph.D.

Bachelor of Arts in Biology (1990)
State University of New York at Oneonta
Department: Biology


Skills:
Biochemistry: 1- and 2-D SDS PAGE and Immunoblotting, Apoptosis Assaying, Radioligand-Receptor Assaying, Antibody Microprobe Technique for detecting neuropeptide release, Flow Cytometry
Chemistry: High-Pressure Liquid Chromatography (HPLC)
Molecular Biology: Nucleic Acid Isolation, UV-VIS Spectrophotometry, Primer Design, Mutagenesis, RT-PCR, Differential Display, Vector Design/Construction, Cloning, Nucleic Acid Hybridization, Sequencing, In situ Hybridizations,
Cytology: Cell Culture- Transfections, Adenovirus Infection, Migration Assays, In vitro Angiogenesis Assays
Microscopy and Histology: Light, Dissecting, Fluorescent, Confocal and Electron Microscope Methods. Tissue Cryosectioning, Immunohistochemical and Immunofluorescent Staining.
Animal Techniques: Rodent Surgery- Cannulation, Tracheotomy, Laminectomy, thoracotomy, Coronary Artery Occlusion (CoAO), Electroneuromodulation, and Perfusion.
Computer Skills: Bioinformatic Analysis: Gene bank searches, BLAST, Alignment, Prediction and Sequence analysis.
Molecular Biology software: Gene Runner, Vector NTI, ClustalX, WinGene, Chromas.
Protein Chemistry Software: ProtLab, Viewer Lite, ExPAS
Biostatistics: Minitab and SigmaPlot Image editing software: Adobe Photoshop
Basic Laboratory Skills: Use of Beta and Gamma Counters, pH Meters, Analytical Balances and other common laboratory equipment.


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