An advanced research position in biochemical engineering or bioprocess
An advanced research position in biochemical engineering or bioprocess
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An advanced research position in biochemical engineering or bioprocessing Resume


Desired Industry: Biotech SpiderID: 5564
Desired Job Location: Edison, New Jersey Date Posted: 2/22/2006
Type of Position: Full-Time Permanent Availability Date: 10/01/2006
Desired Wage: 65000
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, Less Than 25%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Objective:
Summary:

Experienced, enthusiastic and adaptive process engineer offers superior research and development skills in biotechnology.
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Target Position:

An advanced research position in chemical engineering, biochemical engineering or bioprocess engineering where I can use creativity, adaptiveness and organization acumen to positively benefit my employer.
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Target Company

A leading and customer-centered chemical, pharmaceutical or biotechnology company with a wide range of products, and a collaborative culture that encourages people in maximizing their potential.


Experience:
September 2003 to current
Research assistant
Department of Chemical Engineering,
Oregon State University, Corvallis, OR and
Department of Biomedical and Chemical Engineering,
Syracuse University, Syracuse, NY
Conducted research on biosynthesis of the enzyme, manganese peroxidase (MnP), through recombinant DNA technology in bioreactor (project for Ph.D. dissertation).

>>>Researched on protein expression through bench-top fermentations (3L bioreactors) of prokaryotic and eukaryotic systems
>>>Successfully expressed mnp1 gene encoding for MnP in yeast Pichia pastoris
>>>Optimized cultivation process to achieve a 40-fold increase in MnP activity
>>>Developed and optimized the process for MnP concentration including multiple steps such as centrifugation, ultrafiltration, dialysis, and freeze drying
>>>Developed a kinetic model to describe yeast cell growth and enzyme production in bioreactor cultivation using cell density, glucose concentration, enzyme activity, dissolved oxygen, and off-gas CO2 and O2 concentrations
>>>Prepared, executed and documented the standard operating procedures (SOPs) for shake-flask, bioreactor, and enzyme concentration processes

August 2001 to June 2004

Teaching assistant
Department of Biomedical and Chemical Engineering,
Syracuse University, Syracuse, NY
Courses: Chemical Engineering Thermodynamics I & II, Chemical Engineering Laboratory I & II, Fundamentals of Heat and Mass Transfer, Mass and Heat Transfer Operations, Chemical Process Control, and Chemical-Reaction Engineering
>>>Assisted professors in teaching and assessing students in chemical engineering courses
>>>Led group discussions and question-answering sessions
>>>Supervised students in lab.
>>>Graded tests and assignments.

August 2000 to July 2001
Product engineer
Dow Corning (Shanghai) Co., Ltd
Shanghai, China.
>>>Constructed the product database
>>>Designed products' labels according to local laws and regulations using the software-Label View 6.0


Education:
August 2001 to August 2006
Syracuse University, Syracuse, NY
Ph.D., Chemical Engineering, GPA 3.8/4.0

September 1994 to June 1998
East China University of Science & Technology (ECUST), Shanghai, China
BS, Chemical Engineering, GPA 3.6/4.0


Affiliations:
Member since 2003, American Institute of Chemical Engineers


Skills:
>>>Bioreactor operations and fermentation process development, including scale-up and optimization;
>>>Yeast, bacteria and mammalian cell cultivations;
>>>Protein expression and production through recombinant DNA technology;
>>>Protein recovery and purification, including centrifugation, precipitation, lyophilization, filtration (TFF / UF / DF) and chromatography (HIC / affinity / HPLC);
>>>Protein stability test and characterization, including SDS-PAGE, UV spectrophotometry;
>>>Gene recombination, cell electroporation, gel electrophoresis;
>>>Experimental design, process evaluation, data collection and statistical analysis;
>>>Preparation, execution and documentation of the standard operating procedures (SOP) for cell cultivations;
>>>Economic feasibility analysis of fermentation process;
>>>Computer skills, including Word, Excel, Powerpoint and MatLab;
>>>Project management, scientific and technical writing.


Additional Information:
Publications in preparation
1. Fei Jiang, Puapong Kongsaeree, Rose Charron, Curtis Lajoie, Haowen Xu, Gary Scott, and Christine Kelly. Production and separation of manganese peroxidase from heme amended yeast cultures
2. Fei Jiang, Puapong Kongsaeree, Curtis Lajoie and Christine Kelly. Biosynthesis and stability of recombinant manganese peroxidase
3. Fei Jiang, Curtis Lajoie and Christine Kelly. Kinetic analysis of recombinant manganese peroxidase production in Pichia pastoris

Presentation Accepted
4. Christine J. Kelly, Fei Jiang and Curtis Lajoie. Recombinant Expression, Stability and Purification of the Lignin Oxidizing Enzyme Manganese Peroxidase. 2006. Paper #69128. AICHE ĘC Nov. 2006 Annual Meeting.


Reference:
Available upon request.


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