Research Assistant - Biotech Resume Search
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Research Assistant Resume


Desired Industry: Biotech SpiderID: 51156
Desired Job Location: San Francisco, California Date Posted: 5/23/2011
Type of Position: Full-Time Permanent Availability Date: Aug 01, 2011
Desired Wage: 50,000$
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, 50-75%
Highest Degree Attained: Masters Willing to Relocate: Yes


Objective:
A challenging position that will test the
best of my ability


Experience:
Research Assistant at the University of
Texas Health Science Center at Tyler
Aug 2009 to present
•Successfully led projects in the
expression and purification of Par
proteins in Mycobacteria. Expert in
Affinity, Gel filtration, Ion-Exchange,
HPLC and FPLC via AKTA purifier. Studied
and characterized proteins by 2D Gel
electrophoresis, SDS PAGE. Led the team
in sample preparation,
ultracentrifugation
(sucrose/glycerol/CsCl gradient),
antibody purification, dialysis,
immunoprecipitation. Efficient in
evaluating purification yield and
trouble-shooting approaches.
•Successfully cloned, ligated and
transformed nearly 40 genes into
recombinant vectors, well experienced in
design of primers and vectors,
Restriction Digestion and Restriction
Mapping. Sufficient experience in
isolation and purification of DNA and
RNA (plasmid prep) from bacterial and
mice cells.

•Developed ELISA and Western Blotting
successfully for the quantitation of
IgG, IgA and IgM classes of
immunoglobulin, against the soluble
antigens of a Mycobacterium (BCG) in
sera from patients with tuberculosis.
Skillful in the application of Indirect,
Sandwich and Competitive ELISA
techniques.

•Employed QPCR and RT- PCR for gene
expression and quantification of
partitioning genes in Mycobacteria and
transcription to mRNA. Studied Gene
expression analysis by site directed
mutagenesis. Sufficient experience in
working with viral, bacterial and
mammalian expression systems.

•Well-trained in mammalian cell culture
techniques. Skillful and efficient in
maintaining aseptic technique,
optimizing media characteristics,
adapting cell to serum-free environment,
passaging, freezing and storage,
recovering frozen stocks, counting
viable cells.

•Performed Southern Blotting to detect
cancer markers in mice. Isolated and
purified DNA and RNA from bacterial and
mice cells. For gene delivery in H114
mouse cells, employed liposome-mediated
transfection, electroporation,
microinjection methods, to study the
effect of TNF-α on IL8 mRNA in cancer
studies

•Well-experienced in maintaining the
quality of the data produced according
to cGMP regulations, flexible in working
in team environments, efficient in data
interpretation and graphic data
presentation, with appropriate
statistical analysis, successful in
problem-solving and trouble-shooting
approaches. Efficient lab manger,
maintained and documented data in lab
notebooks and ebooks, gave several
presentations in research seminars,
participated in journal club meetings.

Senior Scientist Dept. of
Pharmacy, Andhra University, India
May 2008- June 2009
•Well-trained in protein analysis by
UV/Vis spectroscopy-A280 method, dye-
binding assays (e.g., the Bradford or
Lowry method). Determined the Km, Vmax,
rate and standard curves from the data
obtained. Skillful with flow cytometry
and FACS analysis of cell surface and
markers.

•Employed QPCR for studying the amino
acid change in Pyrococcus horikoshii by
site directed mutagenesis. The mutation
was confirmed by denaturing gel gradient
electrophoresis and by sequence analysis
by comparison with the P. horikoshii
genome sequence.

•Successfully cloned, ligated and
transformed genes into recombinant
vectors, well experienced in design of
primers and vectors, Restriction
Digestion and Restriction Mapping.
Sufficient experience in isolation and
purification of DNA and RNA (plasmid
prep) from bacterial and mice cells. .
Sufficient experience in working with
viral, bacterial and mammalian systems.

•Developed hands on experience in
learning all common biotechnology
practices, pH testing of solutions,
preparation of media and buffers, growth
and maintenance of cell cultures.

•Gained skill on Isolation of mRNA from
various mouse tissues, Nothern blotting,
PCR-basic techniques, primer design,
cloning PCR products, mutagenic PCR,
Real time PCR (Taqman probes & SYBR
Green)

• Collaborated with team members to
develop antibiotic resistance strains in
actinomycetes. Prepared cell media and
buffers required for laboratory

Clinical Research Co-coordinator
King George Hospital, India
March 2007-September 2008
•Completion of case report forms,
verifying data in response to sponsor
queries, obtaining and updating patient
study status

•Verification of Patient screening,
eligibility and enrollment criteria,
verification of informed consents,
accessing source documents from care web
and updating them on the files.

•Extraction of adverse and serious
adverse events, grading and attribution
if any and reporting and updating the
adverse event and SAE information as per
sponsor and IRB/FDA
regulations/Guidelines, Preparing for
Monitor visits including close out

•Participating in the site initiation
visits and regular clinical data
management team meetings to discuss
study updates with investigator and
other clinical team members.

Bioinformatics Intern Indian
Institute of Chemical Technology, India
Jan 2007- March 2008
•Identified more than 2000 disease
target proteins and created a druggable
targets database based on an
understanding of molecular pathways for
easier structure-based drug design, to
create molecules that can bind to the
active site of the targeted enzyme. By
using important databases such as NCBI,
Entrez, PubMed, SwissProt, UniProt and
PDB target proteins with available
crystal structures were identified.

•Was able to visualize target proteins
with appropriate molecular modeling
software, such as VMD, RasMol, PyMOL,
Jmol, MDL Chime, QuteMol etc. A recent
desktop software addition is Sirius.
Now, we are trying to upload this
database online by using TomCat, MySql
softwares.






Education:
Stephen. F. Austin State University
(Graduating Aug 2011)
• MS Biotechnology. Scholarship
holder GPA 3.88 on 4.
• Thesis: Par proteins in M.tb
establish a bridge between DNA
replication initiation and cell
division(Currently, waiting for
publication).
• To study the effect of TNF-Alpha
on IL8 mRNA in cancer studies
• President: Indian Student
Association

Andhra University, Andhra Pradesh, India
(2004-2008)
• Bachelor of Science in
Biochemistry
• Overall topper of the
Biochemistry Department with a GPA of
3.8 on 4. 0 scale; President of
Students of Sai- Old students alumni;
Won several debate, elocution and
singing competitions; School Captain for
girls
• Has given performances in Indian
Classical Dance and participated in
Carnatic Indian Music Festivals.


Skills:
Computer languages:C, C++, Java
Foreign languages:English, Hindi, Telugu


Additional Information:

Dr.Murty Madiraju, Professor
Biochemistry
BMR, UTHCT, 11937,US Highway 271
mmadiraju@uthct.edu
Tel: 903-877-2890
Tyler, Texas 75708, United States


Dr. Michael Pangburn, Professor
University of Health Science Center at
Tyler (UTHCT)
michael.pangburn@uthct.edu
11937,US Hwy-271
Tyler, Texas-75708, United States

Dr. K. Venkateswarlu
Head of the Department of Neurology
King George Hospital (KGH)
Visakhapatnam-530002
India
Email:kvlneuro@hotmail.com
Tel: 91-891-2718179


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