|Desired Industry: Biotech
|Desired Job Location: newark, California
||Date Posted: 5/18/2011
|Type of Position: Full-Time Permanent
||Availability Date: 05/24/11
|Desired Wage: 65000
||U.S. Work Authorization: Yes
|Job Level: Experienced with over 2 years experience
||Willing to Travel: Yes, Less Than 25%
|Highest Degree Attained: Doctoral
||Willing to Relocate: No
Bioassay & Cellular Assay Development
Regulation of Cellular Signal Transduction and Gene Expression
Mechanism of Drug Action
Nuclear / Membrane Receptor Function in diverse physiological and pathological conditions – cancer, cell proliferation, viral infection, inflammation, immune response, stress response, endothelial dysfunction, fibrosis
Chemist, Thermo Fisher Scientific- Fremont, CA, Since Feb 2011
In charge of designing and optimizing a species-specific ELISA protocol to conduct quantitative analyses of Thermo Fisher Scientific antibodies applicable to IHC- IVD (In vitro Diagnostics) as well RUO (Research Use Only)
Involved in routine QC testing of IHC antibodies (Hands on experience of Paraffin-imbedded-tissue based Immune Histo-Chemistry using autostainer) that requires in depth understanding of GMP, GLP & Regulatory procedures
Research Associate Scientist, Roche Pharmaceuticals- Virology, Palo Alto, CA, April 2010 – August 2010
In charge of designing and implementing various strategies and techniques to develop protocols for the successful identification of target(s) for a lead HCV inhibitor compound. The findings will initiate and further its mechanism of action studies. Successfully established a filter-based radio-ligand binding assay protocol
Scientist II, Capstone Therapeutics, Tempe AZ, Jan 2008 – October 2009
Successfully conducted the assessment of functional mechanism of action of Chrysalin® / TP508 (company’s novel synthetic peptide) as a potential therapeutic candidate for vascular dysfunction. The research involved Characterization of phosphorylation of signaling proteins such as endothelial nitric oxide synthase & MAPK activated by TP508 treatment of primary human aortic endothelial cells (HAECs) & Optimization of a protocol for the detection of drug-induced Nitric oxide in primary HAECs and successfully identified and analyzed a cellular response downstream of activation of eNOS, known to influence vascular dysfunction
Was instrumental in the completion of TP508 bioassay development project, one of the company’s crucial goals set for the year 2008
Optimized ELISA and QPCR conditions for the detection of connective tissue growth factor (CTGF) and alpha smooth muscle actin (alpha-SMA) as markers of fibrotic differentiation
Designed and conducted experiments using normal dermal fibroblasts, hypertrophic scar and keloid fibroblasts to understand the mechanism of action of AZX100- a novel 24 amino acid phospho-peptide, in the treatment or prevention of hypertrophic scarring
Hired, mentored and managed two research assistants to execute research aimed at understanding mechanism of action of TP508 and AZX100
Postdoctoral Fellow, UCSF, San Francisco CA, Oct 2002 – Dec 2007
Obtained highly competitive and prestigious National Research Service Award from the National Cancer Institute, NIH (2003-2006). As the sole and principal investigator, determined specific objectives, estimated pitfalls and designed alternative approaches to initiate a challenging genetic screen to identify novel regulators of Liver receptor homologue (LRH-1) in breast, liver and endometrial cancer cells & identified NCoA-62 as a novel co-regulator of LRH-1 mediated target gene expression.
Contributed to a pharmaceutical collaboration with Glaxo SmithKline to test the role of pharmacological agents synthesized as possible LRH-1-ligands in cancer proliferation. Collaborated on other research projects conducted in the laboratory involving post-translational modification of nuclear receptors and mentored three summer training students.
Demonstrated effective time management, executed experiments within the duration of fellowship, performed data analyses and interpreted the results for a manuscript ready for submission.
Education & Fellowships
Post-doctoral Fellow (NRSA Award, NIH)
University of California, San Francisco, Department of Physiology, 2003-2006
Ph.D., Biomedical Sciences (Doctoral Fellowship)
University of Toledo, Medical Sciences, Department of Pharmacology and Therapeutics, 2002
Master of Science, Biochemistry
G.S. Medical College, University of Mumbai, India, 1997
Bachelor of Science, Biochemistry
Bachelor of Science, Chemistry
Sophia College, University of Mumbai, India, 1995
Mechanism of Heat Shock Signaling and Glucocorticoid Receptor Cross-Talk: Repression of Heat Shock Transcription Factor-1 (HSF1) by Glucocorticoid Receptor (GR)
Affiliated to The Endocrine Society since 2001 & The American Society for Cell Biology since 2008
 S. A. Wadekar, D. N. Derkach, K. B. Perkins, E. Rousseau, C. M. Dreiza, J. Cheung-Flynn, H. C. Ramos, T. Ugarova, and M. R. Sheller. RGD-dependent binding of TP508 to integrin αvβ3 mediates cell adhesion, migration and induction of nitric oxide. Thrombosis and Haemostasis, 2010 May 27;104(2)
 Wadekar SA, Lee M, Hur E and Ingraham HA. Nuclear co-activator NcoA-62 interacts with and enhances Liver Receptor Homologue-1-mediated target gene expression (Manuscript in preparation)
 Lee MB, Lebedeva LA, Suzawa M, Wadekar SA, Desclozeaux M and Ingraham HA. The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification. Molecular Cell Biology, 2005; (5): 1879-90.
 Wadekar SA, Li D and Sanchez ER. Agonist-activated glucocorticoid receptor inhibits binding of heat shock factor 1 to the heat shock protein 70 promoters in vivo. Molecular Endocrinology, 2004; (3): 500-8.
 Jones TJ, Li D, Wolf IM, Wadekar SA, Periyasamy S and Sanchez ER. 2004 Enhancement of glucocorticoid receptor-mediated gene expression by constitutively active heat shock factor 1. Molecular Endocrinology, 2004; (3): 509-20.
 Wadekar SA, Li D, Periyasamy S and Sanchez ER. Inhibition of Heat Shock Transcription Factor by Glucocorticoid Receptor. Molecular Endocrinology, 2001; 15(8): 1396-1410.
 Li D, Wadekar SA, Periyasamy S and Sanchez ER. Glucocorticoid Receptor and Heat Shock Transcription Factor Cross-talk- Review Article. Current Topics in Steroid Research: Research Trends, 2000; 3:117-125.
 Steroid Hormone Regulation and Function - Steroid Receptor Signaling Mechanism in Disease and Physiology, San Francisco State University, Department of Chemistry, 2005
 Co-regulation of Glucocorticoid receptor function and stress response, National Institute of Virology, Pune, India, 2000
 Annual ASCB Meeting- San Francisco, CA - 2008
 Keystone Symposia, Nuclear Receptors- Banff, AB, Canada - 2006
 Endocrine Meeting- San Diego, CA – 2005
 Keystone Symposia, Nuclear Receptors- Snowbird, UT – 2002
 Annual Pharmacology Research Symposia, Wayne State University- MI – 2002
 Endocrine Meeting- Denver, CO – 2001
 Keystone Symposia, Nuclear Receptors- Steamboat Springs, CO – 2000
Awards and Honors
 Post-doctoral Fellowship Award (2003-2006) Ruth L. Kirschstein National Research Service Award (NRSA) National Institute of Cancer (NCI) / National Institute of Health (NIH)
 Doctoral Fellowship Award (1997-2002) University of Toledo Medical Sciences (formerly Medical College of Ohio)
CELLULAR AND MOLECULAR BIOLOGY TECHNIQUES
Primary- and Immortalized cell culture, Generation of stably-transfected or transiently-transfected cells using constitutively-active or inducible expression vectors, Reporter gene expression assays (beta galactosidase, luciferase) performed using Microplate Readers M2, M5, Emax, SpectraMax, Immunohistochemistry, IHC-QC support (in a regulated GMP environment) Immuno-fluorescence, Mammalian two-hybrid assay, Co-immunoprecipitation (IP) and Chromatin IP, Cell-adhesion assay, 3-D culture and Endothelial tube formation assay, Cell proliferation assay, Apoptotic cell-death assay, FACS, Bacterial culture for large and small-scale plasmid DNA preparation, DNA ligation and sub-cloning, PCR, RTPCR, QPCR, Site-directed mutagenesis, SiRNA, Yeast two hybrid screen
ELISA, Protein purification, HPLC, SDS PAGE, Western blotting, GST-pull down assay, Radio-ligand Binding Assays, Co-expression and interaction assay using GATEWAY Vectors for protein expression, EMSA, In vitro transcription and translation assay, Chemiluminiscent assay for Nitric oxide detection
Research data management and statistical analyses, Working knowledge and implementation of GLP, GMP. Proficiency in Microsoft Office, Adobe Photoshop, Canvas, Prism, Sigma Plot, Endnote, Protein/DNA Sequencing and Real Time PCR/SiRNA primer/oligo design software such as DNA-Star, Primer Express and OligoEngine, Plate Reader software Softmax-Pro, Working knowledge of automated platforms for robotic plate handling
Successful scientific writing of research grants and publications and Proficiency in biomedical search tools including Entrez Pubmed and Medline
Excellent analytical, communication and interpersonal skills
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