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Molecular Biology Resume

Desired Industry: Biotech SpiderID: 50479
Desired Job Location: Rockville, Maryland Date Posted: 4/28/2011
Type of Position: Full-Time Permanent Availability Date:
Desired Wage:
U.S. Work Authorization:
Job Level: Experienced with over 2 years experience Willing to Travel:
Highest Degree Attained: Willing to Relocate:

Work in a reputed company in the areas of Molecular Biology and Immunology where I can contribute to the best of my skills as well as enhance my career.

Postdoctoral fellow - 1.5 years
Research Scientist - 1.5 years

PhD - Cell and Molecular Biology
MS - Biochemistry

Flow cytometry (FACS) for surface and cytokine markers
Xenograft tumor studies with prostate and metastatic cancer, in-vitro cytotoxicity/ viability assay
Monoclonal antibody: Hybridoma fusion, Lymphocyte isolation and cloning
ELISA: Blood serum and antibody analysis, antigen-specific antibody assay, Experienced in developing and troubleshooting
Immunoassay: Lateral flow assay for identification of specific bacteria, experience in assay development and troubleshoot
Antibody purification: Chromatography techniques, dialysis, spectroscopy
Experienced in Protein/antibody conjugation, Gold nano-particle production
Antigen extraction/purification from bacteria (E.coli-O157, Salmonella, Listeria)

Molecular Biology
Site directed mutagenesis, Primer design
Transfection (retro-viral and non-viral), Electroporation (Nucleofection) methods, transduction, Luciferase reporter assay
Immunoprecipitation, SDS-PAGE, Western blotting
PCR, Real-time quantitative PCR, multiplex-PCR
Southern blotting, agarose gel electrophoresis
DNA/RNA isolation/purification, Plasmid sub-cloning, restriction enzyme digestion
Genome sequencing and annotation, NCBI (BLAST) and other cDNA/protein databases
Protein isolation/quantification, Silver staining, Coomasie staining
RNA isolation from frozen and paraffin embedded tissue sections
Hematoxylin/Eosin staining, Acridine orange staining

Cell culture
Mammalian cell culture, Aseptic techniques, cell line maintenance, subculturing
Hybridoma cell line cloning, cell count/viability, cryo-preservation
Cytokine analysis, Tumor cell isolation, in-vitro cytotoxicity assay

Animal work
Mice: Experienced in laboratory mice work
Handling laboratory mice, retro-orbital, peritoneal, subcutaneous injections
Bone-marrow reconstitution, lymphocyte extraction/purification, Immuno-staining
Xenograft tumor studies, bacterial infection studies
Splenectomy, tumor necropsy, body weight and tumor volume measurements
Animal tissue sectioning using cryostat and microtome, RNA isolation

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