Associate Scientist - Biotech Resume Search
Associate Scientist - Biotech Resume Search
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Associate Scientist Resume


Desired Industry: Biotech SpiderID: 50101
Desired Job Location: Rockland, Maryland Date Posted: 4/18/2011
Type of Position: Full-Time Permanent Availability Date: 5/2/11
Desired Wage: 50000-75000
U.S. Work Authorization: Yes
Job Level: New Grad/Entry Level Willing to Travel: Yes, 25-50%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Objective:
I am looking for a research position in
industry to further develop my career and
research experience. I would eventually
like to move into Field Application
Science.


Education:
University of California, Davis GPA:
3.78
Ph.D. in Microbiology, September 2010
Dissertation Topic: Understanding the
Assembly of the TOR complexes 1 and 2
and the TOR signaling pathways in
Saccharomyces cerevisiae.
Thesis Advisor: Ted Powers
University of Washington, Seattle GPA:
3.67
Bachelor of Science in Microbiology with
distinction, June 2004
Bachelor of Science in Biochemistry,
June 2004


Skills:
Familiar with PCR and molecular
cloning, including creating novel
expression vectors for use in both E.
coli and S. cerevisiae.
Versed in protein over-
expression, purification, and
immunoprecipitation in both E. coli and
S. cerevisiae.
Experience with mammalian cell
culture techniques.
Practiced in fluorescence
microscopy techniques including
staining.
Experienced at evaluating
ability of bleeds or purified antibodies
to recognize proteins by western.
Practiced designing
immunoprecipitation assays to purify
protein for a variety of purposes.
In-depth understanding of
protein analysis by Western, PAGE, and
concentration assays (Bradford and BCA).
Some experience with flow
cytometry.


Additional Information:
RESEARCH EXPERIENCE
Doctoral Research, UC Davis (June 2006-
present)
Created chimeric TOR proteins in
order to examine the structure and
function of the two yeast TOR kinases,
homologs of the human mTOR protein
important in cancer development.
Discerned specific protein
sequences important for determining the
ability of the TOR proteins to modify
other proteins.
Analyzed ability of TOR-complex
specific antibodies to recognize members
of the complexes.
Worked to get results and meet
project goals ahead of conference dates
and grant submission deadlines.

Group Project, UC Davis (January 2008-
August 2010)
Worked with a post-doctoral
scholar to create and manage a long-term
research project to identify targets for
modification by the yeast YPK1 and YPK2
kinases, modified by TOR2.
Used multiple techniques to
purify active YPK1 and YPK2 for use in
enzyme assays.
Designed an immunoprecipitation
assay to purify potential targets of the
YPK proteins.
Worked with advisor to maintain
necessary collaboration with a lab at
the Scripps Research Institute.
Recruited junior graduate
students, undergraduates, and research
technicians to the team.
Consulted with advisor and
postdoctoral researcher to determine
project direction.
Mentored graduate students and
undergraduates in project design and
research methods.
Encouraged students to work
collaboratively to get results under a
strict deadline.

Undergraduate research project,
University of Washington (June 2003-June
2004)
Studied the metabolism of the
methane-producing bacteria,
Methanococcus maripaludis.
Mastered techniques to
manipulate DNA and perform metabolic
analyses on mutant bacteria.

Awarded a full-year Mary Gates research
scholarship ($4500).
NIH MCB Training Grant recipient, two
consecutive years (amount of full
stipend and tuition for graduate
student)


Reference:
Available upon request.


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