Bioanalytical Scientist, LC MS Scientist - Biotech Resume Search
Bioanalytical Scientist, LC MS Scientist - Biotech Resume Search
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Bioanalytical Scientist, LC/MS Scientist Resume


Desired Industry: Biotech SpiderID: 31860
Desired Job Location: San Diego, California Date Posted: 12/22/2009
Type of Position: Full-Time Permanent Availability Date: Jan 2010
Desired Wage: 85000
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, Less Than 25%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Objective:
Accomplished, results-oriented professional interested in working in a bio-analytical research field. Expert in the structural characterization and quantitation of proteins, glycans, lipids and small molecules using cutting edge mass spectrometric techniques. Demonstrated experience in chromatographic method development/validation for pharmaceutical drugs, drug intermediates, raw materials under GLP/FDA regulations.

I am a highly motivated bioanalytical scientist with hands on experience in research and managing complex interdisciplinary team projects focused on proteomic methods and technology development for research in life sciences. My doctoral research involves the development of 157 nm photofragmentation technique for the first time to glycans/lipids structural characterization and also acquired special skills in purification, conjugation, immobilization, chemical modification of biomolecules. I successfully designed and conducted various analytical experiments and organic synthesis processes for the biomolecular structural characterization.


Experience:
• Outstanding 6+ years track record of successful experiences in developing and applying the state-of-the-art proteomics approaches to identify, quantify and characterize proteins and their modifications (glycosylations and phosphorylations) in the biological samples (with the use of Spectrum mill, Mascot and Discoverer software¡¦s).

• As a technical expert I regularly use specialized analytical techniques (Agilent Trap, Thermo Finnigan LTQ, Triple Quad, ABI/SCIEX 4000 QTRAP and Bruker MALDI TOF/TOF ultrafleXtreme with Eksigent 1D nano LC and Agilent LC systems) for proteins, glycans and lipids structural characterization, impurities ¡Vknown/unknown identification and trouble shooting the related technical issues.

• A strong background in chromatographic method development of various biological compounds. Provided support for new LC/MS system installation, preventative maintenance, troubleshooting and minor repairs of analytical scientific equipments.



• Established and achieved quantization methods (MRM and SRM modes) for small lipid molecules and metabolites using 4000 QTRAP mass spectrometer. Additional expertise in biological sample preparation (from tissues, cell lysates and tumors), protein purification, enrichment, fractionation techniques and protein enzymatic digestion.

• Provided bio-analytical support for drug discovery, biomarker analysis and identification of proteins during antibody pull-down assay with the help of SDS-PAGE Gel and western blot techniques.

• Independently determine experimental design, data evaluation, formulation of relevant and scientifically based conclusions, and coordinate/present studies with technical groups.


Education:
PhD in Analytical Chemistry 2002-2008
Laboratory of Prof. James P. Reilly
Indiana University, Indiana, USA

Thesis: ¡§Applications of 157 nm Light Photodissociation for the Structural Characterization of Isomeric Glycans and Lipids¡¨


M.S., General Chemistry 1992-1994
(Physical, Organic and Inorganic Chemistry)
Madras University, Chennai, India.

DCPIC 1994-1995
Diploma in Chemical Process Instrumentation and Control
Annamalai University, Chidhambaram, India.

B. Sc. General Chemistry 1989-1992
(Physical, Organic, Inorganic, Analytical and Pharmaceutical Chemistry)
Madras University, Chennai, India.


Affiliations:
AWARDS AND ACHIEVEMENTS

„« Associate Instructor Best Teaching Award for undergraduates at Indiana University, USA, 2004

„« All India rank holder at GATE [General Aptitude Test in Engineering] ¡V 1994, India

„« Jawaharlal Nehru Talent Award during B. Sc course work.


Skills:
• 157 nm laser light was successfully utilized for the first time to characterize glycans and lipids inside a linear ion trap.

• Developed and demonstrated that the laser can be a powerful tool for the structural characterization of isomeric complex glycans and lipids.

• Designed AP-MALDI and nano-ESI ion sources and successfully coupled with bimolecular photofragmentation experiments.

• Developed and validated sensitive and selective analytical methods for pharma drug products and intermediates using IR, UV-VIS, DSC, NMR, CE, GC-MS and HPLC-MS instrumental techniques.

• A working knowledge of chemistry, stability and degradation, and biophysical techniques, and ability to apply associated principles, theories, and concepts to formulation development.

• Developed broad range of wet chemical analysis for drug molecules, raw materials and intermediates. Evaluated and applied new technology to analytical methods development.

• Presented technical information about assigned projects at meetings and seminars and obtained theoretical and practical understanding of the full range of drug development process.

• Contributed to the implementation of reviewing and verifying data in laboratory notebooks and technical reports. Supervise hiring, training and coaching of 6 junior chemists in my group.

• Maintained and enforced applicable government regulatory, safety, occupational health, and environmental requirements.

• Performed systematic analysis of active pharmaceutical ingredients at various stages, including in-process/intermediate/final products using TLC, HPLC, FTIR, autotitrators, UV-Vis spectrophotometer and polarimeter.

• Provided updates to production/QA regarding progress and issues. Ensured that projects were conducted under GLP guidelines and the FDA guidance and were correctly documented for global submission.

• Applied analytical method validation for new market/research products and successfully handled the regulatory audits like GLP and US-FDA.

• Prioritized workloads and maintained a strong internal and external network. Demonstration of enthusiasm, creativity, initiative, problem-solving skills, track record of tangible productivity, significant contribution to the team and company goals.

• Participated and performed in product development teams to provide technical support for manufacturing, in-process control, quality assurance and analytical R&D that compliance with GLP/GMP regulations.

• Regularly analyzing raw materials, drug intermediates and final pharma drug products (includes tablets, capsules, ointments, injections and liquid orals) as per in house standard operating procedures (SOP) and international pharmacopeia standards. Ensured proper maintenance of instruments including calibration, cleaning and record maintenance.

• Writing day-to-day analytical reports and co-coordinating with production/QA. Involved in reviewing technical documents and effectively manage both internal and external resources assigned to my project(s).

• Established strong working relationship with analytical development and other cross-functional groups with in and outside the company.


Additional Information:
PUBLICATION LIST

1. Devakumar A.; Dillon R.; Greig M.; and Bagrodia S. ¡§Investigation LC/MS Analysis of Phosphoinositol Phosphates¡¨, 2009 (Submitted in Anal Chem.).

2. Nilsson C. L.; Dillon, R.; Devakumar, A.; Shi, S. D-H.; Greig, M.; Rogers, J. C.; Krastins, B.; Rosenblatt, M. M.; Major, M.; Kaboord, B. J.; Sarracino, D.; Rezai, T.; Prakash, A.; Lopez, M.; Ji, Y.; Lang Jr, F. F.; Colman, H. C.; and Conrad, C. A. ¡§Quantitative Phosphoproteomic Analysis of STAT3/IL-6/HIF1£\ Signaling Partners in NSC11 Glioblastoma Stem Cells¡¨, In Press. J Proteome Res. 2009.

3. Devakumar, A.; Mechref, Y.; Kang, P.; Novotny, M. V.; Reilly, J.P. ¡§Direct Identification of Isomeric N-Glycan Structures by Laser-Induced Photofragmentation¡¨, J. Am. Soc. Mass Spectrom. 2008; 19, pp 1027-40

4. Devakumar, A.; O¡¦Dell, D.K.; Walker, J.M.; and Reilly, J.P. ¡§Structural Analysis of Leukotriene C4 isomers using Collisional Activation and 157 nm Photodissociation¡¨, J. Am. Soc. Mass Spectrom.2008; 19, pp 14-26

5. Devakumar, A.; Mechref, Y.; Kang, P.; Novotny, M. V.; Reilly, J.P. ¡§Laser-Induced Photofragmentation of Neutral and Acidic Glycans inside an Ion-Trap Mass Spectrometry¡¨, Rapid Commun. Mass Spectrom. 2007; 21, pp 1452-1460.

6. Devakumar, A.; Thompson, M.S.; Reilly, J.P. ¡§Fragmentation of Oligosaccharide ions with 157 nm Vacuum ultraviolet light¡¨, Rapid Commun. Mass Spectrom. 2005; 19, pp 2313-2320.


Reference:
Available upon request.


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