Research Associate - Biotech Resume Search
Research Associate - Biotech Resume Search
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Research Associate Resume


Desired Industry: Biotech SpiderID: 27849
Desired Job Location: Sunnyvale, California Date Posted: 7/20/2009
Type of Position: Full-Time Permanent Availability Date: 07/21/09
Desired Wage:
U.S. Work Authorization: Yes
Job Level: New Grad/Entry Level Willing to Travel: No
Highest Degree Attained: Bachelors Willing to Relocate: No


Objective:
Seeking a biological research or laboratory position in the biomedical and/or biotech industries.


Experience:
Affymetrix, Fremont, CA
Feb – Jun 2009
Research Associate, Research and Development: Responsible for performing genetic and immunological experiments and analyzing results on customer samples. Responsible for supporting new product development. Also responsible for the establishment of stable cell lines.
• Conducted Procarta Cytokine, DC and Transcription Factor Plex assays to evaluate biological samples
• Performed Quantikine Porcine, TNF-alpha and Transcription Factor ELISAs to analyze samples
• Purified antibodies by using desalt columns, Protein G Plus and the Melon Gel Purification Kit
• Biotinylated mouse antibodies and calculated molar ratios
• Conjugated microspheres to human and mouse antibodies for analysis
• Prepared buffers, bead mixtures, diluents, detection antibody mixtures and SAPE
• Transfected cell lines with a luciferase reporter plasmid and isolated stable transfectant clones
• Assessed reporter gene induction by performing luciferase assays and monitoring reporter stabilities
• Tested and developed new Procarta products. Wrote protocols and updated SOPs
• Recorded detailed laboratory observations, procedures and results in a laboratory notebook
• Created detailed laboratory protocols using the Bioplex software
• Responsible for calibrating and repairing the Luminex machine

Baxter, Hayward, CA
Mar 2008 – Jan 2009
Laboratory Associate, Quality Control Immunology: Responsible for conducting biological, chemical and physical analyses on pharmaceutical samples.
• Performed ELISA, SDS-PAGE, Bradford assay, protein concentration absorption, chromogenic functionality assay and other experiments on samples
• Analyzed assay results of biologics, drugs and pharmaceutical samples
• Prepared buffers, staining solutions, diluents and cleaning solutions for each experiment
• Analyzed and interpreted data using the Biotek ELX808 plate reader, the Beckman DU-800 spectrophotometer, and the molecular dynamics densitometer SI
• Calculated the average, standard deviation, coefficient of variation and percent recovery of samples
• Completed test forms & detailed lab reports. Documented test procedures & interpreted test results
• Ensured compliance with FDA, GLP, QSR and cGMP regulations
• Maintained a minimal error rate for each experiment as required by SOPs
• Performed laboratory and manufacturing audits and updated SOPs
• Performed equipment maintenance, calibration and repair

Affymetrix, South San Francisco, CA
Feb - Mar 2008
Research Assistant: Responsible for washing, staining and scanning GeneChip Mapping 500K probe arrays. Also responsible for bleaching the fluidics stations and running the 500K Fluidics machines.
• Prepared wash and stock buffers. Measured the pH and conductivity of each buffer
• Prepared and analyzed the anti-streptavidin solution and array holding buffer
• Primed the fluidics station by programming the 500K Fluidics machines
• Made the stain buffer, SAPE and antibody solution mixes for staining arrays
• Washed and stained the 500K probe arrays using the 500K Fluidics machines
• Processed the solutions by pipetting and centrifuging the array samples
• Scanned the 500K probe arrays using the GeneChip Scanner 3000 7G laser
• Performed fluidics station bleaching by using the GeneChip Operating Software

Stanford Genome Technology Center, Stanford School of Medicine, Palo Alto, CA
Jan - Feb 2008
Research Assistant: Responsible for conducting emulsion PCR and 454 pyrosequencing experiments. Also responsible for maintaining an inventory of reagents and recording detailed laboratory procedures.
• Performed emulsion PCR and 454 pyrosequencing experiments using yeast and algae DNA
• Amplified single strands of DNA to millions of strands by programming PCR thermocycler machines
• Segregated each DNA capture bead to a microreactor to amplify DNA by emulsion PCR
• Selected amplified DNA capture beads by using magnetic particle collectors
• Programmed thermocycler machines to anneal sequencing primers to amplified DNA
• Prepared the packing and enzyme beads and loaded the reagents into the sequencing cassette
• Launched the sequencing runs by programming the Genome Sequencer FLX Instrument
• Maintained an inventory of the emulsion PCR and 454 pyrosequencing reagents
• Documented and updated detailed laboratory protocols for emulsion PCR and 454 pyrosequencing

Intuitive Surgical, Sunnyvale, CA
Dec 2007 - Jan 2008
Product Quality Assurance Analyst: Responsible for testing, cleaning and analyzing monopolar electrocautery shears for precision, durability and dexterity.
• Operated the da Vinci surgical system. Tested shears for precision by cutting meat samples
• Performed tests that involved snapping, blunt dissections and cautery to test for durability
• Analyzed the tips, covers and cables of the instruments to test for tensile strength
• Autoclaved and sterilized the instruments in enzymatic solution using the ultrasonic cleaning machine

Stanford Research Institute, Menlo Park, CA
Oct - Nov 2007
Laboratory Technician, Environmental Health and Safety: Responsible for scanning and documenting chemicals at SRI’s R & D divisions.
• Scanned and organized chemicals by room and building
• Entered chemical barcodes into SRI’s computer database
• Documented chemical names, properties and manufacturing information


Education:
Bachelor of Science, Molecular Biology, Sept 2007
University of California, San Diego, CA, GPA 3.0


Skills:
Molecular Biology
o electrophoresis, restriction enzyme digest, nucleic acid & recombinant DNA plasmid purification
o emulsion PCR, 454 pyrosequencing, Transcription Factor ELISA, Transcription Factor Plex

Biochemistry
o protein & antibody purification, A280, DC & Bradford, Northern/Southern/Western Blotting
o Procarta Cytokine assay, TNF-alpha & Quantikine Porcine ELISAs, conjugation, biotinylation

Cell Culture
transfection, induction, trypsinization, making frozen stock, luciferase assay

Other
o operating robotic surgical systems, sewing heart tissue, isolating veins and arteries
o chromogenic assay, HPLC chromatography, NMR & IR spectroscopy, C/C++ & Java programming


Additional Information:
Mathematics Award, Language Arts & History Award, Art Design Award, Drawing & Painting Award


Reference:
Available upon request.


Candidate Contact Information:
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