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Scientist Resume


Desired Industry: Biotech SpiderID: 27430
Desired Job Location: King of Prussia, Pennsylvania Date Posted: 6/24/2009
Type of Position: Full-Time Permanent Availability Date:
Desired Wage:
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel:
Highest Degree Attained: Doctoral Willing to Relocate:


Objective:
--


Experience:


PROFESSIONAL EXPERIENCE

University of the Sciences in Philadelphia
Department of Biological Sciences - Philadelphia, PA
TEACHING POST DOCTORAL FELLOW 2008-PRESENT
- Acquire in-depth knowledge of the adaptive phenomenon at molecular level wherein mammalian kidney cells are exposed to high osmolarity as a consequence of renal concentrating mechanism
- Conduct extensive research to investigate the roles of heat shock proteins 27, 70, 60 as well as p38 MAP kinase in osmotic stress pathway in the kidney cell lines
- Facilitate lecture and laboratory classes; teach introductory level of General Biology Laboratory course to biology and pharmacy undergraduate students in Fall 2008
- Assume a pivotal role in course planning and management of BS 103 / 130 for repeat biology students
- Work closely with undergraduate and graduate students to provide direction in research projects

University of Delhi
Interdisciplinary Center for Plant Genomics and Department of Plant Molecular Biology - New Delhi, India
POST DOCTORAL RESEARCH ASSOCIATE 2006-2007
- Proactively involved in identification and characterization of the role of OsSAP (Oryza sativa stress associated protein) gene family in biotic stress in indica rice using quantitative real time PCR
- Engaged in administering various stress treatments wherein wounding, pathogen produced elicitors, salicylic acid, jasmonic acid, cholic acid, and hydrogen peroxide were given and the expression profile of all the eighteen members of SAP gene family were analyzed by real time PCR

University of Delhi
Department of Plant Molecular Biology - New Delhi, India
DOCTORAL STUDENT 2000-2005
- Studied engineering transgenic resistance against rice tungro disease; utilized multiple approaches consisting of both protein mediated strategies as well as RNA interference
- Employed different strategies against RTBV and RTSV and discovered some techniques to develop competency; further tested transgenic lines for tungro resistance at field level to determine if resistance genes can be more introgressed into other agronomically useful varieties
- Focused on standardization of regeneration and genetic transformation of agronomically important indica rice variety ADT 39
- Optimized carbohydrate sources and concentration, and supplementing cytokinins, auxin and amino acids to establish a system for embryogenic calli production , transformation, and regeneration
EDUCATION

DOCTOR OF PHILOSOPHY (PH.D) IN PLANT MOLECULAR BIOLOGY
Interdisciplinary Center for Plant Genomics and Department of Plant Molecular Biology
University of Delhi South Campus - New Delhi, India - 2005

MASTER OF SCIENCE (M.SC.) IN BIOTECHNOLOGY
Indian Institute of Technology - Roorkee, India - 2000



Education:

EDUCATION

DOCTOR OF PHILOSOPHY (PH.D) IN PLANT MOLECULAR BIOLOGY
Interdisciplinary Center for Plant Genomics and Department of Plant Molecular Biology
University of Delhi South Campus - New Delhi, India - 2005

MASTER OF SCIENCE (M.SC.) IN BIOTECHNOLOGY
Indian Institute of Technology - Roorkee, India - 2000


Skills:

QUALIFICATIONS PROFILE

Focused, innovative, and performance-driven professional equipped with proven expertise in numerous scientific approaches with focus on molecular biology. Dedicated individual; enthusiastic to pursue a challenging career as a scientist to further develop management, communication, and troubleshooting skills while achieving career progression. Possesses in-depth knowledge of deploying diverse experimental methods and cutting-edge technologies to deliver high quality products. Experienced in providing primary leadership to diverse group to deliver peak performance and achieve common goal. Utilizes solid communication and interpersonal skills in working collaboratively with culturally diverse people.


TECHNICAL PROFICIENCY

Basic Molecular Biology PCR Amplification, Gene Cloning, Genomic DNA and Total RNA Isolation, Sequencing, Southern Hybridisation, Northern Hybridisation, siRNA Isolation, Protein Isolation and Purification, Western Hybridisation, RFLP, GUS Assay, Quantitative Real Time PCR, Rice and Arabidopsis Tissue Culture and Transformation, Confocal Microscopy, Viral Infection Assays, Animal Cell and Tissue Culture
Software MS Windows, ABI Prism/ Stratagene Real-Time PCR, Vector NTI, Gene Runner, MAPdraw, sequence databases for rice , arabidopsis, mouse and human


Additional Information:

PROFESSIONAL TRAINING

- Presented a poster entitled ‘Roles of p38MAPK and heat shock proteins 27 and 70 in osmotic stress in renal and blood cells’ by Priyanka Patel, Himani Tyagi, Ashwini Khanna, Suzanne K. Murphy and Margaret R. Kasschau at 48th annual meeting of American Society for Cell Biology on December 13 to 17 2008 in San Francisco, California
- Presented a poster entitled ‘ Engineering resistance against tungro using genes derived from rice tungro bacilliform virus and rice tungro spherical virus’ by Himani Tyagi, S. Rajasubramaniam and Indranil Dasgupta ’ during the International Symposium on Rice: From Green Revolution to Gene Revolution on October 4 to 6 2004 at the Directorate of Rice Research, Rajendra Nagar, in Hyderabad, India
- Presented a poster entitled ‘Genetic engineering of rice for resistance against rice tungro spherical virus and rice tungro bacilliform virus using multiple approaches’ by Himani Tyagi, S. Rajasubramaniam, G. Uma, M.V. Rajam and Indranil Dasgupta’ at the 9th National Rice Biotechnology Network Meeting on April 15 to 17 2004 at the NASC Complex in New Delhi, India
- Presented a poster entitled ‘Investigations on the molecular organization of rice tungro viruses from India and development of transgenic rice for viral resistance’ by Nandita Nath, Rekha Joshi, Saloni Mathur, S. Rajasubramaniam, Himani Tyagi, Nitin Dutt, and Indranil Dasgupta at 8th National Biotechnology Network meeting, 21-25 October 2001, Aurangabad, India


MEMBERSHIPS

- Qualified all India Entrance Test with 13th rank for Admission to Master of Science (M.Sc.) in Biotechnology conducted by Indian Institute of Technology, Roorkee, INDIA in the year 1998 for the academic session 1998-2000
- Received scholarship during M.Sc. from the Department of Biotechnology, Government of India, during academic session of 1998 to 2000
- Qualified National Eligibility Test (NET) conducted by University Grants Commission and Council of Scientific Research, New Delhi, India, 1999 providing eligibility for lectureship and receipt of fellowship

PUBLICATIONS

- Himani Tyagi, S. Rajasubramaniam and Indranil Dasgupta (2007) “Regeneration and Agrobacterium - mediated transformation of a popular indica rice variety ADT39” Current Science, Volume 93, Number 5 September, pages 678-683
- Himani Tyagi, S. Rajasubramaniam, M.V. Rajam and Indranil Dasgupta (2008) “RNA interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants.” Transgenic Research, October 17 (5), pages 897-904
- Hitesh Kathuria, Jitender Giri, Himani Tyagi, Akhilesh K. Tyagi (2007) “Advances in Transgenic Rice Biotechnology” Critical Reviews in Plant Sciences, Volume 26, Issue 2 March , pages 65 – 103



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