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Scientist Resume

Desired Industry: Biotech SpiderID: 13704
Desired Job Location: White Plains, New York Date Posted: 7/4/2007
Type of Position: Full-Time Permanent Availability Date: 8-1-07
Desired Wage: 60000
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, 25-50%
Highest Degree Attained: Doctoral Willing to Relocate: No

I want to stay in the NYC metro north area and hope to find a job within 1.5 hours commute to my home. Ideally, I would like to get involved in (anti-terrorism, neurodegenerative disease or bacterial antibiotic resistance research or broadly novel therapeutics. Additionally, I hope the workplace will offer both team research as well as some independent research. Also it will be nice to work where scientific growth is encouraged by support for advanced training courses as well as at meetings & conferences. It would be beneficial if the workplace fostered lively discussion/education in-house in a positive environment.

NYU School of Medicine - Department of Environmental Medicine
Post-Doctoral Fellow (NIH Institutional Training Grant) 7/05-present
Pulmonary Toxicology Laboratory: Investigating post-Inhalational therapies for acute ricin poisoning using antibodies/intrabodies as well as inhibitors of MAPK, JNK and ERK pathways to lessen the toxic sequelae of ricin (RCA60) in the mouse lung.

Immunology & Cell Biology Laboratory Courses: Teaching Assistant 1/97-12/00
Responsible for independently teaching laboratory coursework and scientific writing skills to undergraduate & graduate students in a writing intensive laboratory class.

Veterans Affairs Medical Center: Laboratory Technician: 10/89-12/93
Neurobiology Lab
Performed surgeries to sever the optic chiasm of normal and myelin deficient rats. Examined the optic nerve for re-growth in controls and animals given fetal brain tissue transplants by analyzing fixed brain sections by both light and electron microscopy.
Pulmonology Lab
Correlated the induction of genes and proteins necessary for removal of superoxide radicals formed in cells exposed to hyperoxia or treated with chemical inducers of oxygen radicals.
Hematology Lab
Quantified and compared levels of Heavy and Light Ferritin in serum from healthy and ill men Measured cytokine production in cells as a result of secondary signals from arachidonic acid metabolism and growth factors on lymphocytes.

State University of New York at Albany Ph.D.: Molecular Biology awarded 5/05
State University of New York at Albany MS.: Cell/Molecular Biology awarded 5/97
Cornell University B.S.: Biological Sciences awarded 5/89
State University of New York at Cobleskill A.S.: with Honors (Deanís List graduate) awarded 5/87

University at Albany: 9/96-12/04
Doctoral Thesis Research Project
Identify in vitro transcription conditions allowing transcription from the fis promoter (fisP). Correlated the cis and trans factors important for fisP transcription in vitro with the in vivo changes in log phase growth milieu of E. coli in rich media.

Doctoral Thesis: Pratt, T.S., 2005, College of Arts & Science, Department of Biology. Thesis Collection, State University of New York at Albany. Transcription Study of the Escherichia Coli Fis Promoter: Characterization of Factors Affecting Transcription In vitro and Examination of Alternative Transcription Initiation Sites In vitro and In vivo.

University at Albany: 8/93-8/96
Masters Thesis Research Project
Uncovered in vivo factors and DNA elements responsible for the dramatic peak of the fis promoter's transcriptional activity during the exponential growth phase of E. coli.

Masters Thesis: Pratt T.S.,1996, Masterís Thesis, College of Arts & Science, Department of Biology. Thesis Collection, State University of New York at Albany. In vivo Regulation of the fis Promoter in Escherichia coli.


Tissue Culture, Stereotaxic Surgery, Tissue Transplantation, Electron & Light Microscopy, Photomicrography, Perfusion, Micro-Dissection, Tissue Fixation, Microtomy, Immunohistochemistry, Protein Assays, ELISA, SDSĖPAGE, Western Blots, Enhanced-Chemiluminescence, PCR & PCR Directed Mutagenesis, RT-PCR, Northern Blots, Southern Blots, DNA Cloning, Maxam-Gilbert And Sanger DNA Sequencing, Primer Extensions, Gel Shift Assays, DNase I Footprinting, β Galactosidase Assays, P1 Phage Transduction, In Vitro Transcription Assays, Molecular Dynamics Instruments (Phosphorimaging & Densitometry), Scintillation and Imaging Beta Counters, DNA Microarray Analysis, Created Databases for Laboratory Strain and Oligonucleotide Collections, Extensive Knowledge Of Macintosh And Windows OSís.

Additional Information:

Meranda D. Bradley, Michael B. Beach, Jason de Koning, Timothy S. Pratt and Robert Osuna. Global Effects of Fis on Escherichia coli Gene Expression During Growth. Manuscript Submitted 4-07 to: Microbiology. Status: Accepted.

Timothy S. Pratt, Martha L. Hale, Chad J. Roy, and Kam-Meng Tchou-Wong. Oropharyngeal Aspiration of Ricin as a Lung Challenge Model for Evaluation of the Therapeutic Index of Antibodies against Ricin A-Chain for Post-Exposure Treatment. Manuscript Submitted 1-07 to: Experimental Lung Research: Status: Accepted pending revisions.

Kimberly A. Walker, Prabhat Mallik, Timothy S. Pratt, and Robert Osuna. The Escherichia coli fis promoter is regulated by changes in the levels of its transcription initiation nucleotide CTP. J. Biol. Chem., 2004 Dec 3;279(49):50818-28. Epub 2004 Sep 22.

Mallik P, Pratt TS, Beach MB, Bradley MD, Undamatla J, Osuna R. Growth phase-dependent regulation and stringent control of fis are conserved processes in enteric bacteria and involve a single promoter (fisP) in Escherichia coli. J. Bacteriol. 2004 Jan;186(1):122-35.

Pratt, T.S., T. Steiner, L. Feldman, K. Walker, and R. Osuna. Deletion Analysis of the fis Promoter
Region in Escherichia coli: Antagonistic Effects of IHF and Fis. J. Bacteriol Vol 179:20:Oct 1997, pp.6367-6377.


Pratt T. S., Mallik P, Walker K., and Osuna R. Transcription activity of the Escherichia coli fis
promoter is strongly influenced by its initiating NTP (CTP), salt concentration, and DNA topology. Poster for: The American Society of Microbiology - General Meeting, May 2002, Salt Lake City Utah.

Pratt, T., P. Weigele, U. Kies, H. Reeg, T. Steiner, and R. Osuna. Function and Regulation of the E. coli DNA Binding Protein Fis. Presented at The Molecular Genetics of Bacteria and Phages Meeting, August 22-27, 1995, Cold Spring Harbor NY.

Pratt, T., U. Kies, H. Reeg, P. Weigele, and R. Osuna. Regulation of the E. coli DNA Binding Protein Fis. Poster for: American Society for Microbiology, Eastern New York Branch meeting, November 11, 1994, Schenectady, NY.

D. Pasquale, T. Pratt, G. Chikkappa. Lipoxygenase products are required for GM-CSF Production in T-cells. Blood, Vol. 82, Issue 10, p.a503,1993.

E. Ortof, T. Pratt, C.K. Csiza, and K.D. Barron. Fetal Tissue Transplantation in a Myelin Deficient Rat. Presented at The Meeting of the Society for Experimental Neuropathology November 1992, Toronto.

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