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Scientist Resume


Desired Industry: Biotech SpiderID: 78883
Desired Job Location: Palm Beach Gardens, Florida Date Posted: 2/28/2017
Type of Position: Full-Time Permanent Availability Date:
Desired Wage: 75000
U.S. Work Authorization: Yes
Job Level: Management (Manager, Director) Willing to Travel: Yes, 25-50%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Objective:
Highly analytical, innovative and goal-oriented research scientist, offering hands-on experience in research and development within the areas of cell-based assay, high-throughput and high-content drug screening, transporter/channel kinetics, immune-neural interaction, and protein recombination and purification. Adept in cellular and molecular biotechnology techniques, along with bioinformatics to expose molecular mechanisms of innate immune and brain memory management system. Excellent in performing multiple tasks, while staying focused on prioritized tasks. Articulate communicator with demonstrated proficiency in handling independent and collaborative assignments. Well-versed in English and Mandarin Chinese. Currently hold United States Permanent Residency.


Experience:
The Scripps Research Institute, Neuroscience Department, Jupiter, FL
Postdoctoral Fellow in Neuroscience May 2011–Present
Transcriptome analysis of dorsal striatum of Bipolar Disorder patients (RNA isolation, ribosomal RNA removal, NGS library construction).
Lead a multidisciplinary team in screening 400 memory-related factors from 3500 brain enriched genes, particularly 42 memory-suppressor-genes to provide a whole picture of the molecular profile of brain memory management and active forgetting systems.
Perform bioinformatics analysis as local blast algorithm to discover human orthologous neurodisease-causing genes in larger database.
Employ analytical skills in conducting research on inhibitors of memory suppressor genes as potential drugs for amnesia improvement, which involves generating HEK293-SLC22A (human transporter) permanent cell line; designing fluorescent substrate; and developing cell-based fluorescent inhibition assay for high-throughput and -content screens.
Obtain knowledge of substrates and inhibitors of SLC22 through cell-based (mouse primary neuron and engineered HEK293, and CHO cell) studies of enzyme/transporter kinetics (Km, Vmax, and Ki) with BMG CLARIOstar® plate reader.
Generate SLC22A knockout mouse, and characterize its spatial memory performance, anxiety and depression level utilizing Morris water maze, open field test and forced swim test.
Demonstrate partial rescue of memory-related phonotypes in SLC22- deficiency mouse through oral administration of neurotransmitters/precursors.
Utilize bioinformatics tools to perform comparison of gene expression profiles of senescence-sensitive neurons in young and aged animals; as well as evaluate shared molecular basis of immune and nervous systems.
Generate 11 recombinant proteins in both bacterial and mammalian system and purify them to measure their activities.
Identify and study the function of regulator of G protein signaling (RGS) in animal brain memory.
Manage the generation and purification of antibodies against SLC22A transporter and RGS protein.
Notable Accomplishment:
Successfully discovered a novel transporter SLC22 as memory suppressor gene (Alzheimer’s drug target) that grabbed the attention of various newspapers and websites, including ScienceDaily and NewsToday, following publication to reputable journal, Neuron.

University of Missouri, School of Biological Sciences, Kansas City, MO
Postdoctoral Fellow in Innate Immunity May 2010–May 2011
Perform biochemical studies on mechanisms of antibacterial peptides (defensins, crustins, anti-lipopolysaccharide factors).
Apply keen eye for detail in scrutinizing a new factor of potential LPS signal pathway–lipopolysaccharide-induced TNF-alpha factor (LITAF) of C. elegans, Drosophila, and Manduca.

Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
Founder Scientist Jun 2009–May 2010
Developed new clinical diagnostic reagents with dry chemistry technology.
Contributed to an integrated dry chemistry analyzer project in multidisciplinary team (instrumentation, optics, microelectronics, material science, and software).
Worked closely with Medical Instruments of Chuangchun Institute of Optics, Fine Mechanics Corporation.

Research Project

2005–2009
Project Name: The cDNA library, EST analysis and prophenoxidase system of Eriocheir sinensis
Role: Ph. D. candidate
Company/Institution Name: Chinese Academy of Sciences
Overview/Objective: Decoding of transcriptome of one unique species, Eriocheir sinensis with only few sequences available in public databases.
Generated 7535 high-quality EST sequences and established the molecular and genetic foundation for E.sinensis.
Conceptualized a strategy for challenging animals with pathogen mixture prior to sample collection, which led to remarkable increase of immune gene abundance from 3% to 13% in cDNA library.
Elucidated mRNA temporal expression profiles, protein functions of 10 genes of the arthropod unique immune system (proPO-system).
Identified a novel thymosin-like protein, and demonstrated that the corresponding recombinant protein enhances the proliferation of human hepatocellular carcinoma and mouse neuro-2a cell.
Notable Accomplishment:
Earned recognition as one of the first scientists to discover SIBD in crustaceans; thus disclosing its bi-functional roles in immune and neuroendocrine systems and providing solid support to long-term speculation that immune and neuroendocrine systems have a common origin.


Education:
Doctor of Philosophy in Innate Immunity: May 2009
Key Laboratory of Pathogen, University of Chinese Academy of Sciences, Beijing, China

Bachelor of Science in Biotechnology: Jun 2004
Hefei University of Technology, Hefei, China
Thesis: Comparison of Genetic Difference between the Bacterial Blight Resistant and the Wild Strain using RAPD and SSR markers


Skills:
Areas of Expertise

DNA/RNA
DNA Cloning | cDNA Library Construction | Overlap & Realtime PCR | Plasmid Construction (AAV) | RNA Isolation | RNAi | Genetic Knockout/Knockdown Animal | NGS Library
Protein/Antibody
Protein Recombination/purification | Antibody Purification | Western Blotting | ELISA
Protein Kinetics
Cell Culture

Cell Culture/Transfection | Cell Line Development | Primary Neuron/ Glia
Reporter Gene Assay | Fluorescent Qualification | Flow Cytometry
Tissue Imaging
Fine Brain Dissection | Cryostat | Immunohistochemistry (IHC)| Brain Functional Imaging Confocal/ Fluorescence Microscopy | Super Resolution Microscopy
Bioinformatics Analysis
EST analysis | Local Blast | ExPASy tools
High-Throughput Tech

Drosophila/Mouse Behavior
BMG Clariostar® Plate Reader | Biomek Liquid Handling Automation
Eppendorf epMotion Liquid Handling System | InCell 6000 Automation Imaging System
Morris Water Maze | Forced Swim Test | Open Field Test | Appetitive/Aversive Memory Assay | Long-term Massed/Spaced Conditioning
Technical Proficiency
Prism | Illustrator | Vector NTI/MacVector | Mega | Perl Language


Additional Information:
Internship

Beijing Institute of Genomics, Beijing, China
Intern 2004–2005

Professional Development

Chinese National Computer Rank Examination Certificate: Grade: 3A
Bioinformatics Analysis Training: Beijing Genomics Institute
Neural Differentiation of iPS Cells | 3D and 2D Cell Culture |IACUC| Radioactive Material Safety: Scripps Florida
HACCP in Chinese: Hefei University of Technology

Awards and Honors

Second-Class Award of Science and Technology Progress Prize, Chinese National Bureau of Oceanography: 2014
Second-Class Innovation Award, Chinese National Bureau of Oceanography: 2009
Excellent Doctoral Dissertation of Chinese Academy of Sciences: 2010
First-Class Graduate Scholarship: 2007; 2006; 2005
Excellent Organizer, 1st National Postgraduate Forum of Marine Biology: 2007

Activities

Member of Society of Neuroscience | Member of Genetics Society
Vice President–Student Union, Institute of Oceanology, Chinese Academy of Sciences (IOCAS)
Committee Member–International Conference on Fisheries & Aquaculture
Member–Toastmaster International | Volunteer Scientist–CASEC | Volunteer Teacher–HFUT

Journals
Gai, Y., Liu, Z., MacMullen C., Davis R. (In preparation) Cell-based fluorescent competitive inhibition HTS screen of human SLC transporter inhibitors and transporter kinetics.
Gai, Y., Liu, Z., Cervantes-Sandoval, I., and Davis, R. (2016). Drosophila SLC22A transporter is a memory suppressor gene that influences cholinergic neurotransmission to the mushroom bodies. Neuron (IF=15), 90(3), 581-595.
Gai, Y., Qiu, L., Song, L., Wang, L., Zhao, J., Mu, C….and Li, L. (2009). A clip domain serine protease (cSP) from the Chinese mitten Eriocheir sinensis with multiple potential functions. Fish and Shellfish Immunol, 27(6), 670-677.
Gai, Y., Wang, L., Song, L., Zhao, J., Qiu, L., Wang, Bo…and Xing, K. (2008). cDNA cloning, characterization and mRNA expression of a pacifistic light chain gene from the Eriocheir sinensis. Fish and Shellfish Immunol, 25(5), 657-663.
Gai, Y., Wang, L., Song, L., Zhao, J., Qiu, L., and Xing, K. (2010). A putative endocrine factor single insulin binding domain protein (SIBD) involved in immune response of Eriocheir sinensis. Fish & Shellfish Immunol, 28 (1), 10-17.
Gai, Y., Wang, L., Zhao, J., Qiu, L., Song, L., Ling, L., and Mu, C. (2009). The construction of a cDNA library enriched for immune genes and the analysis of 7535 ESTs from Eriocheir sinensis. Fish and Shellfish Immunol, 27(6), 684-694.
Gai, Y., Zhao, J., Song, L., Li, C., Zheng, P., Qiu, L., and Ni, D. (2008). A prophenoloxidase from the Chinese mitten Eriocheir sinensis: Gene cloning, expression, and activity analysis. Fish & Shellfish Immunol, 24 (2), 156-167.
Gai, Y., Zhao, J., Song, L., Wang, L., Qiu, L., Ning, X…and Li, L. (2009). Two thymosin-repeated molecules with structural and functional diversity coexist in Eriocheir sinensis. Developmental & Comparative Immunol, 33(7), 867-876.
Gao, D., Vallejo, V.A., He, B., Gai, Y., and Sun, L. (2009). Detection of DNA changes in somaclonal mutants of rice using SSR markers and transposon display. Plant Cell, Tissue and Organ Culture, 98 (2), 187-196.
Kong, P., Wang, L., Zhang, H., Zhou, Z., , Qiu, L., Gai, Y., and Song, L. (2010). Two novel secreted ferritins involved in immune defense of Chinese mitten crab Eriocheir sinensis. Fish & Shellfish Immunol, 28 (4), 604-612.
Li, F., Xu, L., Gai X, Zhou, Z., Wang, L., Zhang, H, Gai, Y.,…and Liang, C. (2013).The involvement of PDGF/VEGF related factor in regulation of immune and neuroendocrine in Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol, 35(4), 1240-8.
Li, F., Wang, L., Qiu, L., Zhang, H., Gai, Y., and Song L. (2012). A double WAP domain-containing protein from Chinese mitten crab Eriocheir sinensis with antimicrobial activities against gram-negative bacteria and yeast. Dev Comp Immunol, 36(1), 183-90.
Li, C., Zhao, J., Song, L., Mu, C., Zhang, H., Gai, Y…and Xing, K. (2008). Molecular cloning, genomic organization, and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis. Developmental & Comparative Immunol, 32(7), 784-794.
Mu, C., Zhao, J., Wang, L., Song, L., Song, X., Zhang, H…Gai, Y., and Cui, Z. (2009). A thioredoxin with antioxidant activity identified from Eriocheir sinensis. Fish & Shellfish Immunol, 26 (5), 716-723.
Mu, C., Zhao, J., Wang, L., Song, L., Zhang, H., Li, C…and Gai, Y. (2009). Molecular cloning and characterization of peroxiredoxin 6 from Chinese mitten crab Eriocheir sinensis. Fish & shellfish Immunol, 26 (6), 821-827.
Mu, C, Zheng, P, Zhao, J, Wang, L, Qiu, L, Zhang, H…and Song, L. (2011). A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol, 31(1), 142-7.
Mu, C., Zheng, P., Zhao, J., Wang, L., Zhang, H., Qiu, L, Gai, Y., …and Song, L. (2010) Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis. Developmental & Comparative Immunol, 34 (7), 734-740.
Walkinshaw, E., Gai, Y., Farkas, C., Richter, D., Nicholas, E., Keleman, K., and Davis, R. (2015). Identification of genes that promote or inhibit olfactory memory formation in Drosophila. Genetics, 199(4), 1173-82.
Wang, L., Ma, Z., Yang, J., Gai, Y., Zhou, Z., Wang, L…and Song, L. (2013). Identification and characterization of a serine protease inhibitor Esserpin from the Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol, 34(6), 1576-86.
Wang, M., Wang, L. Yi, Q., Gai, Y., and Song, L. (2015). Molecular cloning and characterization of a cytoplasmic manganese superoxide dismutase and a mitochondrial manganese superoxide dismutase from Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol, 47 (1), 407-417.
Wang, M., Wang, L., Zhou, Z., Gai, Y., Mu, C., and Song, L. (2013). The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis. International journal of immunogenetics, 40(3), 230-240.
Wang, M., Zhang, D., Wang, L., Gai, Y., Zhou, Z., Zhang, H., and Song, L. (2013). The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein. Electronic Journal of Biotechnology, 16 (2).
Wang, L., Zhang, Y., Wang, L., Yang, J., Zhou, Z., Gai, Y…and Song, L. (2011). A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity. African Journal of Biotechnology, 10 (77), 17678-17689.
Yang, J., Wang, L., Huang, M., Wang, L., Gai, Y., Qiu, L….and Song, L. (2011). An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol, 30(6), 1303-9.
Zhang, Y., Wang, L., Yang, J., Gai, Y., Qiu, L., and Song, L. (2010). The second anti-lipopolysaccharide factor (EsALF-2) with antimicrobial activity from Eriocheir sinensis. Developmental & Comparative Immunol, 34(9), 945-952.
Zhang, Y., Wang, L., Zhao, J., Song, L., Gai, Y., Dong, C…and Qiu, L. (2010). The effects of CpG-C oligodeoxynucleotides on innate immune responses in Eriocheir sinensis (H. Milne-Edwards, 1853). Aquaculture Research, 41 (10), 656-666.
Zheng, P., Wang, H., Zhao, J., Song, L., Qiu, L., Gai, Y.,…and Xing, K. (2008). A lectin (CfLec-2) aggregating Staphylococcus haemolyticus from scallop Chlamys farreri. Fish & Shellfish Immunol, 24 (3), 286-293.
Zhang, Y., Zhao, J., Zhang, H., Gai, Y., Wang, L., Li., F…and Song, L. (2010). The involvement of suppressors of cytokine signaling 2 (SOCS2) in immune defense responses of Chinese mitten crab Eriocheir sinensis. Developmental & Comparative Immunol, 34 (1), 42-48.

Presentations

Gai, Y., Liu, Z., and Chakraborty, M. (2015). RNAi screen identified memory suppressor genes in Drosophila. Conference of flies on the beach. Jupiter, FL.
Gai, Y., Liu, Z., Chakraborty, M., and Davis, R. (2014). Solute carrier protein is required in the adult mushroom body neurons for the regulation of Drosophila memory acquisition and memory loss. 44th Annual Meeting of Society for Neuroscience. Washington, DC.
Gai, Y., Liu, Z., and Davis, R. (2013). SLC knockdown enhances memory in Drosophila. Scripps Neuroscience Departmental Research Meeting. West Palm Beach, FL.
Gai, Y., Liu, Z., and Davis, R. (2016). SLC22A transporter is a memory suppressor gene that influences cholinergic neurotransmission to the mushroom bodies. 46th Annual Meeting of Society for Neuroscience (SfN), San Diego, CA.

Patents

Song, L., Mu, C., Wang, L., Gai, Y., Zhao, J., Qiu, L., and Zheng, P. (2010). Patent No. CN 101565703 B, China: SIPO.
Song, L., Mu, C., Wang, L., Gai, Y., Zhao, J., Qiu, L., and Zheng, P. (2009). Patent No. CN 101525617 A, China: SIPO.
Yao, X., Song, L., Wang, W., Wang, L., Gai, Y., Suo, J…and Qiu, L. (2010). Patent No. CN101619102 A, China: SIPO.


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