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Scientist Resume


Desired Industry: Biotech SpiderID: 78043
Desired Job Location: New York, New York Date Posted: 9/14/2016
Type of Position: Full-Time Permanent Availability Date:
Desired Wage:
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: No
Highest Degree Attained: Doctoral Willing to Relocate: No Preference


Objective:
Seeking a biological research position in the biomedical and/or biotech industries


Experience:
Research Scientist
New York University, New York, NY
Apr 2016 – present
• Design experiments for studying of TRPM4 channels in heart em-ploying Western blot, qPCR, cell culture, fluorescent and transmis-sion electron microscopy, RNA-seq analysis, patch-clamp, co-IP, DNA constructs design, mice injections and autopsies.
• Responsible for training of junior staff and students.
• Leading the laboratory journal club.

Postdoc
University of Iowa, Iowa City, IA
Nov 2010 – Mar 2016
• Discovered physiological role of novel endogenous exercise factor Musclin
• Developed ELISA assay for detection a of a novel hormone in blood, developed and validated qPCRs, Western blot, ran multiple biochemistry assays for metabolic markers and hormones, primary myocyte preparation, mitochondria isolation, Seahorse metabolic measurements, indirect calorimetry, activity measurements and physical performance testing in mice, infrared imaging, transmission electron microscopy, image quantification, data statistics, antisense gene silencing.
• Contributed to studies of KATP channels and energy metabolism.
• Supervised junior research staff.
• Completed bioinformatics course.

Scientist
State Research Center of Virology and Biotechnology “Vector”, Novosibirsk, Russia
Sep 2009 – Nov 2010
• Completed an independent project on evolutionary analysis of epi-demiologically significant Flaviviruses
• Reviewed PhD theses and articles.
• Trained junior research staff.
• Finished school on Clinical Laboratory Diagnostics, GLP.

Junior Scientist
State Research Center of Virology and Biotechnology “Vector”, Novosibirsk, Russia ,
Jul 2003 – Sep 2009
• Professionally certified for working in BSL-4 labs.
• Completed project PhD: discovered genetic mutations that affected pathogenicity of Ebola virus employing: virus propagation and purification, infection of guinea pigs, animal autopsy, hematological counting, RT-PCR, sequencing, computational predictions of protein structure.
• Coordinated collaboration between institutions for the project “The study of Vilyui encephalomyelitis: identification of the etiologic agent; development of rational ways of treatment and prophylaxis” funded by Biotechnology engagement program of the US Department of Health and Human Services. Prepared progress reports.

Research Assistant
State Research Center of Virology and Biotechnology “Vector”, Novosibirsk, Russia
Jul 2001 – Jul 2003
• Produced polyclonal antibodies.
• Mastered gene cloning, recombinant protein expression and purification, rabbit immunization, blood sampling, antiserum testing in ELISA and Western blot, antibody affine purification.


Education:
PhD molecular biology
State Research Center of Virology and Biotechnology Vector, Novosibirsk, Russia
2009
MSc (with honors), molecular biology
Novosibirsk State University, Novosibirsk, Russia
2003


Affiliations:
New York University, New York, NY
University of Iowa, Iowa City, IA
State Research Center of Virology and Biotechnology "Vector", Novosibirsk, Russia
Novosibirsk State University, Novosibirsk, Russia


Skills:
EXPERTISE
• Striated muscle physiology
• Metabolism
• Virology

Molecular biology/biochemistry:
• Western blot
• ELISA: assay development of direct and indirect ELISAs (competitive, sandwich), radioimmunoassays
• qPCR: assay development and validation
• Sequencing, RNA-seq analysis
• Recombinant proteins: gene cloning, expression, purification of proteins
• Antibodies: raising of polyclonal antibodies, purification
• Immunoprecipitation/co-immunoprecipitation
• Gel filtration, affine chromatography, solid phase extraction
• Various biochemical tests (metabolic markers , hormones, fatty acids, glucose, glycerol, insulin, glucagon, natriuretic peptides etc.)

Animal and cell culture work:
• Animal immunization, autopsy, blood sampling (mice, guinea pigs, rabbits)
• Cell cultures: primary cell cultures, transfection (viruses, DNA, RNA).
• Anti-sense gene silencing.

Virology:
• BSL-4, 3, 2.
• Propagation and purification of viruses, viral titration, viral DNA/RNA/protein isolation from cell cultures and animal tissues, neutralization test, animal infection.
• Design of custom viral constructs
• Reverse genetic systems (experience with hepatitis C virus in vitro propagation system).

Microscopy:
• Fluorescent, confocal, transmission electron microscopy

Physiology:
• Seahorse assay
• Mice treadmill testing (exercise/exercise tolerance)
• Indirect calorimetry (quantification and analysis of activity, oxygen consumption, heat production etc.)
• Infrared thermal imaging and analysis
• Whole cell and excised patch patch-clamp

Computational:
• Windows, Linux, data quantification, data statistics, RNA-seq analysis, PCR primer and probe design, DNA/RNA/protein databases, design of genetic constructs, evolutionary analysis (phylogenetic reconstruction, estimation of substitution rates, test for selection, molecular clock).


Additional Information:
PATENTS (Russia)
1. Patent of the Russian Federation # 2395576, Jul. 2010; “The strain 1B2 of the Mus musculus L. cells and the strain 7B12 of the Rattus Norvegicus cells – producers of noncompetitive monoclonal antibodies, useful for enzyme-linked immunosorbent “sandwich” system for detection of Zaire Ebolavirus (strain Mayinga) nucleoprotein, and monoclonal antibodies, that were produced by this strains”; authors: Kazachinskaya E.I., Pereboev A.V., Ivanova A.V., Kachko A.V., Subbotina E.L., Chepurnov A.A., Razumov I.A., Loktev V.B.
2. Patent of the Russian Federation #. 2395577 , Jul. 2010; “The strain 4A2 of the Mus musculus L. cells and the strain 1C1 of the Mus musculus L. Cells – producers of noncompetitive monoclonal antibodies, useful for enzyme-linked immunosorbent “sandwich” system for detection of Zaire Ebolavirus (strain Mayinga) VP40 protein, and monoclonal antibodies, that were produced by this strains”; authors: Kazachinskaya E.I., Ivanova A.V., Kachko A.V., Subbotina E.L., Chepurnov A.A., Razumov I.A., Loktev V.B.

PUBLICATIONS
1. Gao Z, Sierra A, Zhu Z, Koganti SR, Subbotina E, Maheshwari A, Anderson ME, Zingman LV, Hodgson-Zingman DM. Loss of ATP-Sensitive Potassium Channel Surface Expression in Heart Failure Underlies Dysregulation of Action Potential Duration and Myocardial Vulnerability to Injury. PLoS One. 2016 Mar 10;11(3):e0151337. doi: 10.1371/journal.pone.0151337. ECollection 2016.
2. Sierra A, Subbotina E, Zhu Z, Gao Z, Koganti SR, Coetzee WA, Goldhamer DJ, Hodgson-Zingman DM, Zingman LV. Disruption of ATP-sensitive potassium channel function in skeletal muscles promotes production and secretion of musclin. Biochem Biophys Res Commun. 2016 Feb 26;471(1):129-34. doi: 10.1016/j.bbrc.2016.01.166. Epub 2016 Jan 30.
3. Subbotina E, Sierra A, Zhu Z, Gao Z, Koganti SRK, Reyes S, Stepniak E, Walsh SA, Acevedo MR, Perez-Terzic CM, Hodgson-Zingman DM, Zingman LV. Musclin is an activity-stimulated myokine that enhances physical endurance. Proc Natl Acad Sci U S A. 2015 Dec 14.
4. Subbotina E, Koganti SR, Hodgson-Zingman D, Zingman LV. Morpholino-driven gene editing: A new horizon for disease treatment and prevention. Clin Pharmacol Ther. 2015 Oct 16. doi: 10.1002/cpt.276.
5. Koganti SR, Zhu Z, Subbotina E, Gao Z, Sierra A, Proenza M, Yang L, Alekseev A, Hodgson-Zingman D, Zingman L. Disruption of KATP channel expression in skeletal muscle by targeted oligonucleotide delivery promotes activity-linked thermogenesis. Mol Ther. 2015 Apr;23(4):707-16. doi: 10.1038/mt.2015.21. Epub 2015 Feb 4.
6. Subbotina EL, Loktev VB. Molecular evolution of West Nile virus. Mol Gen Mikrobiol Virusol (Russia) 01/2014; 29(1):34-41.
7. Zhu Z, Sierra A, Burnett CM, Chen B, Subbotina E, Koganti SR, Gao Z, Wu Y, Anderson ME, Song LS, Goldhamer DJ, Coetzee WA, Hodgson-Zingman DM, Zingman LV. Sarcolemmal ATP-sensitive potassium channels modulate skeletal muscle function under low-intensity workloads. J Gen Physiol. 2014 Jan;143(1):119-34
8. Sierra A, Zhu Z, Sapay N, Sharotri V, Kline CF, Luczak ED, Subbotina E, Sivaprasadarao A, Snyder PM, Mohler PJ, Anderson ME, Vivaudou M, Zingman LV, Hodgson-Zingman DM. Regulation of cardiac ATP-sensitive potassium channel surface expression by calcium/calmodulin-dependent protein kinase II. Biol Chem. 2013 Jan 18;288(3):1568-81.
9. Subbotina EL, Loktev VB. Molecular evolution of the Tick-borne encephalitis and Powassan viruses. Molecular Biology. Mol Biol (Mosk). 2012 Jan-Feb;46(1):82-92.
10. Zhu Z, Burnett CM, Maksymov G, Stepniak E, Sierra A, Subbotina E, Anderson ME, Coetzee WA, Hodgson-Zingman DM, Zingman LV. Reduction in number of sarcolemmal KATP channels slows cardiac action potential duration shortening under hypoxia. Biochem Biophys Res Commun. 2011 Dec 2;415(4):637-41.
11. Subbotina EL, Kachko AA, Chepurnov AA. Genetic determinants of Ebola virus virulence in guinea pigs. Virus Research, 2010 Oct;153(1):121-133.
12. Lee HS, Zhdanova SN, Vladimirtsev VA, Platonov FA, Osakovskiy VL, Subbotina EL, Broytman O, Danilova AP, Nikitina RS, Chepurnov AA, Krivoshapkin VG, Gajdusek DC, Savilov YD, Garruto RM, Goldfarb LG. Epidemiology of Viliuisk encephalomyelitis in Eastern Siberia. Epidemiology. 2010. Jan 21(1). P. 24-30.
13. Sizikova LP, Dadaeva AA, Subbotina EL, Nikitina RA, Shvarts YaSh, Kozlov VA, Chepurnov AA. [The blood picture in Viliuysk encephalitis patients. Sibirskiy Medicinskiy Zhurnal]. 2008. Vol 4.P. 47-49. Russian.
14. Subbotina EL, Chepurnov AA. Molecular mechanisms of Ebola virus reproduction. Vopr. Virusol. 2007. №1. P. 10-16.
15. Dadaeva AA, Sizikova LP, Subbotina E, Chepurnov A. [The hematological and immunological features of Ebola virus infection during passages in guinea-pigs]. Vopr. Virusol. 2006. Vol. 4. P. 32-37. Russian.
16. Subbotina EL, Kachko AA, Chepurnov A. Structural and functional properties of Ebola virus proteins. Vopr. Virusol. 2006. Vol. 6. P. 4-10.


PRESENTATIONS
1. E. Subbotina, A. Sierra, Z. Zhu, Z. Gao, S. Koganti, E. Stepniak, D. Hodgson-Zingman, L. Zingman Musclin – A Novel Exercise Factor. Experimental Biology 2016. San Diego, CA
2. Subbotina E, Sierra A, Zhu Z, Koganti SR, Gao Z, Proenza M, Yang L, Stepniak E, Grumbach I, Hodgson-Zingman DM, Zingman LV Muscle-specific peptide Musclin is essential for muscle adaptation to exercise. 2014 2nd Annual François M. Abboud Cardiovascular Research Center International Symposium. Iowa City, IA. USA. 2014.
3. Subbotina E, Sierra A, Zhu Z, Koganti SR, Gao Z, Proenza M, Yang L, Stepniak E, Grumbach I, Hodgson-Zingman DM, Zingman LV Muscle-specific peptide Musclin is essential for muscle adaptation to exercise. 2014 Health Science Research Week. Iowa City, IA. USA. 2014.
4. Subbotina E, Dadaeva A, Kachko A, Chepurnov A. Molecular determinants of Ebola virus adaptation to guinea pigs. IV congress of Russian Society of Biochemists and Molecular biologists. Novosibirsk. Russia. 2008.
5. Subbotina E, Kachko A, Dadaeva A, Chepurnov A. The experimental study of pathogenicity factors of Ebola virus. Third European Congress of Virology. Nurnberg. Germany. 2007.
6. Subbotina EL, Kachko AV. Dadaeva AA and Chepurnov AA. Experimental study of genetic factors of Ebola virus virulence. III Russian conference “Issues of infectious pathology in Siberia, Far East and Far North”. Novosibirsk. Russia. 2007.
7. Subbotina EL, Kachko AV, Dadaeva AA and Chepurnov AA. Genetic and pathological factors correlating with the alteration of virulence of Ebola virus. XIII International Conference Negative Strand Viruses. Salamanca. Spain. 2006.
8. Subbotina EL, Chepurnov AA. Molecular diagnostics of Ebola hemorrhagic fever. Russian Scientific Conference “Genetic diagnostics of infectious diseases”. Novosibirsk. 2005.


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