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Scientist Resume


Desired Industry: Biotech SpiderID: 63074
Desired Job Location: Wilmington, North Carolina Date Posted: 8/14/2012
Type of Position: Full-Time Permanent Availability Date: sept 1st 2012
Desired Wage: 96000
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, 25-50%
Highest Degree Attained: Doctoral Willing to Relocate: Yes


Objective:
Seeking a position that will offer long term and
financial opportunity. I have acquired a vast amount
of small and large molecule analysis experience and
wish to utilize these skills in a laboratory setting.


Experience:
Professional Experience:
08/2011 08/2012 Post Doctoral Fellow,
Biochemistry, Indiana University
Structural characterization of prokaryotic,
transcriptional regulators involved in copper sensing.
Solution state NMR spectroscopy utilized to assign
protein backbone of structural candidates.
Acquire and interpret data from ESI-LC/MS, MALDI-
TOF, AUC, AA, and gel filtration.
Serve as an in lab technical expert to assist graduate
student in their research projects.
Research new technologies and participate in the peer
review process.

05/2007 07/2011 Post Doctoral Fellow,
Biochemistry, University of British Columbia
Structural characterization of transcription factor
proteins implicated in the development of cancer.
Dynamic and structural studies accomplished utilizing
2D and 3D NMR spectroscopy.
Project involvement included gene cloning, protein
purification, data acquisition, and data analysis.
Structure calculations performed utilizing ARIA and
CYANA programs.
Technical expertise in liquid chromatography: Ni+2
affinity, ion exchange, and gel filtration.
Proficient with recombinant DNA techniques:
insert/overlap PCR, mutagenesis and cloning.
Routine use of instrumentation such as: MALDI-TOF,
Circular Dichroism, HPLC and SDS-PAGE.

01/2001 06/2001 Teaching Assistant, Chemistry,
University of Iowa
Prepared and directed problem solving discussion
groups for premed chemistry students.

06/1994 12/1997 Research Scientist, Reckitt &
Colman
Created analytical methods, researched new
technology, and performed competitor analysis.
Introduced new methods to manufacturing sites and
served as a technical analysis representative.
Accumulated operating and theoretical knowledge in
HPLC, GC, GC/MS, FTIR, IC & AA.

01/1993 05/1994 Quality Control Chemist,
Finished Products, Schein Pharmaceutical


Education:
Education:
06/2001 04/2007 University of Utah, PhD
Biochemistry.
Research focused on the structural and functional
characterization of metalloproteins involved in
cytochrome c oxidase assembly.

Developed and optimized recombinant expression
systems for metalloproteins.
Characterization achieved through spectroscopic
techniques such as NMR, UV-VIS, & AA.
Functional significance of proteins assayed by
monitoring respiration both in vitro and in vivo.
Plasmid construction, mutagenesis, Western Blot,
mitochondrial fractionation, yeast biology
Significant portion of thesis data collected while at
the University of Firenze, Italy.



Additional PhD Accomplishments
Teaching Assistant for Structural Methods Course
with emphasis on MS, NMR, & X-ray.
Developed critical thinking on current literature
through departmental and group Journal Clubs.
Involvement with a diverse research group
supplemented my knowledge of molecular biology.

01/1998 12/2000 University of Iowa, Masters in
Chemistry.
Studies were conducted on the activity and
mechanism of lactoperoxidase.
Paramagnetic NMR and EPR were utilized to study
potential mechanisms and intermediates.
HPLC and Column Chromatography routinely used in
sample preparation.

09/1988 12/1992 University of Delaware, B.S.
Chemistry, Minor Economics


Additional Information:
Grants & Awards:
University of Utah Hematology Training Grant
2002 2003
University of Utah Biological Training Grant
2003 2004
University of Firenze Fellowship
2005 2006


Publications:

Coyne HJ 3rd, Giedroc DP Backbone resonance
assignments of the homotetrameric (48 kDa) copper
sensor CsoR from Geobacillus thermodenitrificans in
the apo- and Cu(I)-bound states: Insights into
copper-mediated allostery. BIOMOL NMR ASSIGN
2012 (submitted)

Coyne HJ 3rd, Soumya D, Okon M, Green SM,
Bhachech N, Graves BJ, McIntosh LP. Auto-inhibition
of ETV6 (TEL) DNA-binding: appended helices
sterically block the ETS domain. J. Mol. Biol. 2012:
421,1, 67-84.

Green SM, Coyne HJ 3rd, McIntosh LP, Graves BJ. DNA
binding by the ETS protein tel (ETV6) is regulated by
autoinhibition and self-association. J Biol Chem.
2010; 285, 24, 18496-504.

Coyne HJ 3rd, Ciofi-Baffoni S, Banci L, Bertini I, Zhang
L, George GN, Winge DR. The characterization and
role of zinc binding in yeast Cox4. J Biol Chem. 2007;
282,12, 8296-8934.

Structures: available at www.rcsb.org
2LF7.pdb
2LF8.pdb
20DX.pdb


Reference:
Available upon request.


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