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Scientist Resume

Desired Industry: Biotech SpiderID: 60490
Desired Job Location: Philadelphia, Pennsylvania Date Posted: 5/14/2012
Type of Position: Contractor Availability Date: Now
Desired Wage:
U.S. Work Authorization: Yes
Job Level: Experienced with over 2 years experience Willing to Travel: Yes, 25-50%
Highest Degree Attained: Doctoral Willing to Relocate:

With a eight years of research &
development experience in molecular
biology and biochemistry, I desire to
develop a career in biopharmaceutical
industry by using my technical,
scientific, leadership, problem solving
and management skills to carry out lab
bench work that meet the business goals
of a cutting edge company.

Research Experience
Epigenetics and chromatin remodeling in
cancer biology 2008-2011
Used in vitro models and cell-
based assays to study cell cycle and
chromatin components after androgen
Examined potential
transcriptional effects using microarray
analysis to provide a genome-wide
picture of chromatin binding and gene
Characterized one of the
chromatin subunit to reveal a function
in prostate cancer using biochemistry,
cell biology and transcriptional
Graduate Ph.D. Research Project
Using southern blot and northern
blot evaluated telomere status and
microsatellite instability liver cancer
and chronic hepatitis patients.
Worked in multicentre phase III
clinical trials of antiviral agents for
hepatitis B & C virus therapy.


Thomas Jefferson University,
Philadelphia, PA
Postdoctoral Fellow in Cancer
Biology, Kimmel Cancer Center
Postgraduate Institute of Medical
Education and Research, Chandigarh,
Ph.D. in Molecular Hepatology
Panjab University, Chandigarh, India
M.S Biochemistry
Kurukshetra Univeristy, India
B.S. Biochemistry
1996- 1999

Technical Skills
Molecular biology: DNA, RNA
isolation, Real-time PCR (qRT-PCR),
Reverse transcriptase PCR, nested PCR,
primers designing, preparation of cDNA
constructs, Designing and radiolabeling
of DNA probes, mouse genotyping
analysis, PCR-genotyping, gene
amplification, agarose electrophoresis
and DNA-PAGE, silver staining, elution
of DNA fragment from gel, Southern and
northern blotting, In situ
Cell biology: Mammalian cell
cultures/cell lines, transfection of
cells (lipofectamine), centrifugation

Protein biochemistry: SDS-PAGE,
native PAGE, western blotting,
immunoassays, EIA, RIA, ELISA, enzymatic
assays, protein purification including
affinity chromatography, gel filtration,
Thin layer chromatography.
Clinical biochemistry: Liver
function tests, Kidney function tests,
Biochemical tests in the sera samples
Histology: Dissection and
tissue fixation, cryosectioning,
paraffin embedding & sectioning, H&E,
antibody Immunostaining.
Genetics & Animal Studies: Mouse
knock-out and knock-in construct design
and strain generation, Survival
surgeries in mice including castration,
renal capsule grafts, Xenograft studies
(SCID/Nude mouse), Harvesting mouse
organs, Retro-orbital/jugular vein/ tail
vein bleeding in mice, Mouse embryo
dissection, Maintaining mouse colonies &
breeding strategies.
Microscopy: Light and
fluorescence microscopy.
Spectroscopy: UV-vis-
spectroscopy, nano-drop.
Pharmacology: Oral dosing, IP,
SC, IM, IV (tail vein) & Orthotopic
injections in animals.
Analytical Chemistry:
Distillation, crystallization
General Laboratory activities :
solutions/ reagent/ buffers preparation,
testing, trouble-shooting, maintenance &
operation of lab equipments, preparation
of reports, summaries, abstracts,
protocols and proper documentation etc.

Additional Information:
I have U.S, employment Authorization and I
can work for any employer without the need
for any visa sponsorship.
I am interested in both contract as well
as full time permanent positions.

Available upon request.

Candidate Contact Information: has chosen not to make contact information available on this page.
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